Effect of autologous adipose tissue-derived mesenchymal stem cells on neovascularization of artificial equine tendon lesions

Philipp Conze; Hans Tm van Schie; Carsten Staszyk; Sabine Conrad; Thomas Skutella; Klaus Hopster; Karl Rohn; Peter Stadler; Florian Geburek; René van Weeren

doi:10.2217/rme.14.55

Effect of autologous adipose tissue-derived mesenchymal stem cells on neovascularization of artificial equine tendon lesions

Philipp Conze, Hans Tm van Schie, Carsten Staszyk, Sabine Conrad, Thomas Skutella, Klaus Hopster, Karl Rohn, Peter Stadler, Florian Geburek, René van Weeren

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

AIMS: To investigate whether autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) treatment of tendon lesions increases neovascularization during tendon healing.

MATERIALS & METHODS: A standardized surgical model was used to create lesions in both front limb superficial digital flexor tendons (SDFTs) of nine horses. Either AT-MSCs or control substance was injected intralesionally 2 weeks post-surgery. Color Doppler ultrasonography of SDFTs was performed at regular intervals. Horses were euthanized 22 weeks post-treatment and SDFTs were harvested for histology.

RESULTS: The color Doppler ultrasonography signal was significantly more extensive at 2 weeks post-treatment and the number of vessels counted on histologic slides was significantly higher at 22 weeks post-treatment in AT-MSC-treated SDFTs.

CONCLUSION: Our findings indicate that AT-MSC treatment has a beneficial effect on neovascularization of healing tendons.

Original language	English
Pages (from-to)	743-57
Number of pages	15
Journal	Regenerative Medicine
Volume	9
Issue number	6
DOIs	https://doi.org/10.2217/rme.14.55
Publication status	Published - Nov 2014

Keywords

adipose tissue-derived
color Doppler ultrasonography
Factor VIII
mesenchymal stem cells
neovascularization
superficial digital flexor tendon
von Willebrand factor

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title = "Effect of autologous adipose tissue-derived mesenchymal stem cells on neovascularization of artificial equine tendon lesions",

abstract = "AIMS: To investigate whether autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) treatment of tendon lesions increases neovascularization during tendon healing.MATERIALS & METHODS: A standardized surgical model was used to create lesions in both front limb superficial digital flexor tendons (SDFTs) of nine horses. Either AT-MSCs or control substance was injected intralesionally 2 weeks post-surgery. Color Doppler ultrasonography of SDFTs was performed at regular intervals. Horses were euthanized 22 weeks post-treatment and SDFTs were harvested for histology.RESULTS: The color Doppler ultrasonography signal was significantly more extensive at 2 weeks post-treatment and the number of vessels counted on histologic slides was significantly higher at 22 weeks post-treatment in AT-MSC-treated SDFTs.CONCLUSION: Our findings indicate that AT-MSC treatment has a beneficial effect on neovascularization of healing tendons.",

keywords = "adipose tissue-derived, color Doppler ultrasonography, Factor VIII, mesenchymal stem cells, neovascularization, superficial digital flexor tendon, von Willebrand factor",

author = "Philipp Conze and {van Schie}, {Hans Tm} and Carsten Staszyk and Sabine Conrad and Thomas Skutella and Klaus Hopster and Karl Rohn and Peter Stadler and Florian Geburek and {van Weeren}, Ren{\'e}",

year = "2014",

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doi = "10.2217/rme.14.55",

language = "English",

volume = "9",

pages = "743--57",

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T1 - Effect of autologous adipose tissue-derived mesenchymal stem cells on neovascularization of artificial equine tendon lesions

AU - Conze, Philipp

AU - van Schie, Hans Tm

AU - Staszyk, Carsten

AU - Conrad, Sabine

AU - Skutella, Thomas

AU - Hopster, Klaus

AU - Rohn, Karl

AU - Stadler, Peter

AU - Geburek, Florian

AU - van Weeren, René

PY - 2014/11

Y1 - 2014/11

N2 - AIMS: To investigate whether autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) treatment of tendon lesions increases neovascularization during tendon healing.MATERIALS & METHODS: A standardized surgical model was used to create lesions in both front limb superficial digital flexor tendons (SDFTs) of nine horses. Either AT-MSCs or control substance was injected intralesionally 2 weeks post-surgery. Color Doppler ultrasonography of SDFTs was performed at regular intervals. Horses were euthanized 22 weeks post-treatment and SDFTs were harvested for histology.RESULTS: The color Doppler ultrasonography signal was significantly more extensive at 2 weeks post-treatment and the number of vessels counted on histologic slides was significantly higher at 22 weeks post-treatment in AT-MSC-treated SDFTs.CONCLUSION: Our findings indicate that AT-MSC treatment has a beneficial effect on neovascularization of healing tendons.

AB - AIMS: To investigate whether autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) treatment of tendon lesions increases neovascularization during tendon healing.MATERIALS & METHODS: A standardized surgical model was used to create lesions in both front limb superficial digital flexor tendons (SDFTs) of nine horses. Either AT-MSCs or control substance was injected intralesionally 2 weeks post-surgery. Color Doppler ultrasonography of SDFTs was performed at regular intervals. Horses were euthanized 22 weeks post-treatment and SDFTs were harvested for histology.RESULTS: The color Doppler ultrasonography signal was significantly more extensive at 2 weeks post-treatment and the number of vessels counted on histologic slides was significantly higher at 22 weeks post-treatment in AT-MSC-treated SDFTs.CONCLUSION: Our findings indicate that AT-MSC treatment has a beneficial effect on neovascularization of healing tendons.

KW - adipose tissue-derived

KW - color Doppler ultrasonography

KW - Factor VIII

KW - mesenchymal stem cells

KW - neovascularization

KW - superficial digital flexor tendon

KW - von Willebrand factor

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DO - 10.2217/rme.14.55

M3 - Article

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SN - 1746-0751

VL - 9

SP - 743

EP - 757

JO - Regenerative Medicine

JF - Regenerative Medicine

IS - 6

ER -

Effect of autologous adipose tissue-derived mesenchymal stem cells on neovascularization of artificial equine tendon lesions

Abstract

Keywords

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