Dynamics and metal exchange properties of C4C4 RING domains from CNOT4 and the p44 subunit of TFIIH

Klaartje Houben, Emeric Wasielewski, Cyril Dominguez, Esther Kellenberger, R. Andrew Atkinson, H. Th. Marc Timmers, Bruno Kieffer, Rolf Boelens

Research output: Contribution to journalArticleAcademicpeer-review


Zinc fingers are small structured protein domains that require the coordination of zinc for a stable tertiary fold. Together with FYVE and PHD, the RING domain forms a distinct class of zinc-binding domains, where two zinc ions are ligated in a cross-braced manner, with the first and third pairs of ligands coordinating one zinc ion, while the second and fourth pairs ligate the other zinc ion. To investigate the relationship between the stability and dynamic behaviour of the domains and the stability of the metal-binding site, we studied metal exchange for the C4C4 RING domains of CNOT4 and the p44 subunit of TFIIH. We found that Zn2+-Cd2+exchange is different between the two metal-binding sites in the C4C4 RING domains of the two proteins. In order to understand the origins of these distinct exchange rates, we studied the backbone dynamics of both domains in the presence of zinc and of cadmium by NMR spectroscopy. The differential stability of the two metal-binding sites in the RING domains, as reflected by the different metal exchange rates, can be explained by a combination of accessibility and an electrostatic ion interaction model. A greater backbone flexibility for the p44 RING domain as compared to CNOT4 may be related to the distinct types of protein-protein interactions in which the two C4C4 RING domains are involved. © 2005 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)621-637
Number of pages17
JournalJournal of Molecular Biology
Issue number3
Publication statusPublished - 10 Jun 2005


  • Metal exchange kinetics
  • NMR15N relaxation
  • RING domain
  • Spectral density mapping
  • Zinc finger
  • cadmium
  • ccr4 not transcription complex subunit 4 protein
  • protein p44
  • protein subunit
  • transcription factor
  • transcription factor IIH
  • unclassified drug
  • zinc finger protein
  • zinc ion
  • article
  • binding site
  • electricity
  • ion exchange
  • metal binding
  • molecular dynamics
  • molecular model
  • nuclear magnetic resonance spectroscopy
  • physical chemistry
  • priority journal
  • protein domain
  • protein protein interaction
  • protein stability


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