Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming

Arie J Hoogendijk; Farzin Pourfarzad; Cathelijn E M Aarts; Anton T J Tool; Ida H Hiemstra; Luigi Grassi; Mattia Frontini; Alexander B Meijer; Maartje van den Biggelaar; Taco W Kuijpers

doi:10.1016/j.celrep.2019.10.082

Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming

Arie J Hoogendijk
, Farzin Pourfarzad
, Cathelijn E M Aarts
, Anton T J Tool
, Ida H Hiemstra
, Luigi Grassi
, Mattia Frontini
, Alexander B Meijer
, Maartje van den Biggelaar
, Taco W Kuijpers

Department of Molecular and Cellular Hemostasis, Sanquin Research, Amsterdam, the Netherlands.
Department of Blood Cell Research, Sanquin Research, Amsterdam University Medical Center (AUMC), University of Amsterdam, Amsterdam, the Netherlands.
Department of Haematology, University of Cambridge, Cambridge CB2 0PT, UK.
National Health Service Blood and Transplant, Cambridge Biomedical Campus, Cambridge CB2 0PT, UK; British Heart Foundation Centre of Excellence, Cambridge Biomedical Campus, Long Road, Cambridge CB2 0QQ, UK.
Department of Blood Cell Research, Sanquin Research, Amsterdam University Medical Center (AUMC), University of Amsterdam, Amsterdam, the Netherlands; Department of Paediatric Immunology and Infectious Diseases, Emma Children's Hospital, AUMC, University of Amsterdam, Amsterdam, the Netherlands. Electronic address: [email protected].

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Human neutrophilic granulocytes form the largest pool of innate immune cells for host defense against bacterial and fungal pathogens. The dynamic changes that accompany the metamorphosis from a proliferating myeloid progenitor cell in the bone marrow into a mature non-dividing polymorphonuclear blood cell have remained poorly defined. Using mass spectrometry-based quantitative proteomics combined with transcriptomic data, we report on the dynamic changes of five developmental stages in the bone marrow and blood. Integration of transcriptomes and proteome unveils highly dynamic and differential interactions between RNA and protein kinetics during human neutrophil development, which can be linked to functional maturation of typical end-stage blood neutrophil killing activities.

Original language	English
Pages (from-to)	2505-2519.e4
Number of pages	15
Journal	Cell Reports
Volume	29
Issue number	8
DOIs	https://doi.org/10.1016/j.celrep.2019.10.082
Publication status	Published - 19 Nov 2019

Keywords

neutrophil development
granule proteins
proteomics
transcriptomics
granulopoiesis

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10.1016/j.celrep.2019.10.082

1-s2.0-S2211124719314044-mainFinal published version, 2.97 MBLicence: CC BY-NC-ND

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Hoogendijk, A. J., Pourfarzad, F., Aarts, C. E. M., Tool, A. T. J., Hiemstra, I. H., Grassi, L., Frontini, M., Meijer, A. B., van den Biggelaar, M., & Kuijpers, T. W. (2019). Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming. Cell Reports, 29(8), 2505-2519.e4. https://doi.org/10.1016/j.celrep.2019.10.082

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abstract = "Human neutrophilic granulocytes form the largest pool of innate immune cells for host defense against bacterial and fungal pathogens. The dynamic changes that accompany the metamorphosis from a proliferating myeloid progenitor cell in the bone marrow into a mature non-dividing polymorphonuclear blood cell have remained poorly defined. Using mass spectrometry-based quantitative proteomics combined with transcriptomic data, we report on the dynamic changes of five developmental stages in the bone marrow and blood. Integration of transcriptomes and proteome unveils highly dynamic and differential interactions between RNA and protein kinetics during human neutrophil development, which can be linked to functional maturation of typical end-stage blood neutrophil killing activities.",

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AU - Tool, Anton T J

AU - Hiemstra, Ida H

AU - Grassi, Luigi

AU - Frontini, Mattia

AU - Meijer, Alexander B

AU - van den Biggelaar, Maartje

AU - Kuijpers, Taco W

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AB - Human neutrophilic granulocytes form the largest pool of innate immune cells for host defense against bacterial and fungal pathogens. The dynamic changes that accompany the metamorphosis from a proliferating myeloid progenitor cell in the bone marrow into a mature non-dividing polymorphonuclear blood cell have remained poorly defined. Using mass spectrometry-based quantitative proteomics combined with transcriptomic data, we report on the dynamic changes of five developmental stages in the bone marrow and blood. Integration of transcriptomes and proteome unveils highly dynamic and differential interactions between RNA and protein kinetics during human neutrophil development, which can be linked to functional maturation of typical end-stage blood neutrophil killing activities.

KW - neutrophil development

KW - granule proteins

KW - proteomics

KW - transcriptomics

KW - granulopoiesis

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JO - Cell Reports

JF - Cell Reports

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ER -

Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming

Abstract

Keywords

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