DNA tattoo vaccination: Effect on plasmid purity and transfection efficiency of different topoisoforms

S.G.L. Quaak; J.H. van den Berg; K. Oosterhuis; J.H. Beijnen; J. Haanen; B. Nuijen

DNA tattoo vaccination: Effect on plasmid purity and transfection efficiency of different topoisoforms

S.G.L. Quaak
, J.H. van den Berg
, K. Oosterhuis
, J.H. Beijnen
, J. Haanen
, B. Nuijen

Dept of Pharmaceutical Sciences

extern

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Recently, DNA tattooing was introduced as novel intradermal administration technique for plasmid DNA (pDNA) vaccines. The aim of this study was to determine if tattooing affects the integrity of pDNA (reduction in supercoiled (SC) content) and whether a change in pDNA topology would affect antigen expression and immune response. We show that 1.) in vitro tattooing of pDNA solutions results in minor damage to pDNA (= 80% SC). (C) 2009 Elsevier B.V. All rights reserved.

Original language	Undefined/Unknown
Pages (from-to)	153-159
Number of pages	7
Journal	Journal of Controlled Release
Volume	139
Issue number	2
Publication status	Published - 2009

Bibliographical note

ISI:000271344200010

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Cite this

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abstract = "Recently, DNA tattooing was introduced as novel intradermal administration technique for plasmid DNA (pDNA) vaccines. The aim of this study was to determine if tattooing affects the integrity of pDNA (reduction in supercoiled (SC) content) and whether a change in pDNA topology would affect antigen expression and immune response. We show that 1.) in vitro tattooing of pDNA solutions results in minor damage to pDNA (= 80\% SC). (C) 2009 Elsevier B.V. All rights reserved.",

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AU - Oosterhuis, K.

AU - Beijnen, J.H.

AU - Haanen, J.

AU - Nuijen, B.

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AB - Recently, DNA tattooing was introduced as novel intradermal administration technique for plasmid DNA (pDNA) vaccines. The aim of this study was to determine if tattooing affects the integrity of pDNA (reduction in supercoiled (SC) content) and whether a change in pDNA topology would affect antigen expression and immune response. We show that 1.) in vitro tattooing of pDNA solutions results in minor damage to pDNA (= 80% SC). (C) 2009 Elsevier B.V. All rights reserved.

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