DNA tattoo vaccination: Effect on plasmid purity and transfection efficiency of different topoisoforms

S.G.L. Quaak; J.H. van den Berg; K. Oosterhuis; J.H. Beijnen; J. Haanen; B. Nuijen

DNA tattoo vaccination: Effect on plasmid purity and transfection efficiency of different topoisoforms

S.G.L. Quaak, J.H. van den Berg, K. Oosterhuis, J.H. Beijnen, J. Haanen, B. Nuijen

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Abstract

Recently, DNA tattooing was introduced as novel intradermal administration technique for plasmid DNA (pDNA) vaccines. The aim of this study was to determine if tattooing affects the integrity of pDNA (reduction in supercoiled (SC) content) and whether a change in pDNA topology would affect antigen expression and immune response. We show that 1.) in vitro tattooing of pDNA solutions results in minor damage to pDNA (= 80% SC). (C) 2009 Elsevier B.V. All rights reserved.

Original language	Undefined/Unknown
Pages (from-to)	153-159
Number of pages	7
Journal	Journal of Controlled Release
Volume	139
Issue number	2
Publication status	Published - 2009

Cite this

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title = "DNA tattoo vaccination: Effect on plasmid purity and transfection efficiency of different topoisoforms",

abstract = "Recently, DNA tattooing was introduced as novel intradermal administration technique for plasmid DNA (pDNA) vaccines. The aim of this study was to determine if tattooing affects the integrity of pDNA (reduction in supercoiled (SC) content) and whether a change in pDNA topology would affect antigen expression and immune response. We show that 1.) in vitro tattooing of pDNA solutions results in minor damage to pDNA (= 80% SC). (C) 2009 Elsevier B.V. All rights reserved.",

author = "S.G.L. Quaak and {van den Berg}, J.H. and K. Oosterhuis and J.H. Beijnen and J. Haanen and B. Nuijen",

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AU - Quaak, S.G.L.

AU - van den Berg, J.H.

AU - Oosterhuis, K.

AU - Beijnen, J.H.

AU - Haanen, J.

AU - Nuijen, B.

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AB - Recently, DNA tattooing was introduced as novel intradermal administration technique for plasmid DNA (pDNA) vaccines. The aim of this study was to determine if tattooing affects the integrity of pDNA (reduction in supercoiled (SC) content) and whether a change in pDNA topology would affect antigen expression and immune response. We show that 1.) in vitro tattooing of pDNA solutions results in minor damage to pDNA (= 80% SC). (C) 2009 Elsevier B.V. All rights reserved.

M3 - Article

SN - 0168-3659

VL - 139

SP - 153

EP - 159

JO - Journal of Controlled Release

JF - Journal of Controlled Release

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