DNA methylation mediates the association between occupational exposures and lung function

Diana Van Der Plaat; Maaike De Vries; Sacha Bastide-Van Gemert; Kim De Jong; Cleo Van Diemen; Ivana Nedeljkovic; Najaf Amin; Hans Kromhout; Roel Vermeulen; Dirkje Postma; Cornelia Van Duijn; Judith Vonk; Marike Boezen

doi:10.1183/1393003.congress-2017.OA2946

Abstract

Introduction: Occupational exposures, such as biological dust, mineral dust and gases/fumes, are associated with lower lung function levels and attribute to 15-20% of all Chronic Obstructive Pulmonary Disease (COPD) cases. Epigenetic mechanisms such as DNA methylation have been suggested to play a role in these associations. Aim: To assess if the association between occupational exposures and lung function (FEV /FVC) is mediated by DNA methylation. Methods: We included 1,561 subjects of the LifeLines cohort with either no, low, or high occupational exposure to biological dust, mineral dust and gases/fumes based on the current or last held job. Associations between the three exposures and 420,938 blood DNA methylation sites (CpGs, Illumina 450K array) were assessed using robust linear regression adjusted for appropriate confounders. Differentially methylated regions (DMRs) were identified using comb-p in python. Mediation of the top site per region was assessed using bootstrapping in R. Results: Using p

Original language	English
Article number	OA2946
Journal	European Respiratory Journal
Volume	50
Issue number	suppl 61
DOIs	https://doi.org/10.1183/1393003.congress-2017.OA2946
Publication status	Published - 1 Sept 2017

Keywords

biological product
endogenous compound
galectin 3
glucocorticoid receptor
adult
bootstrapping
chronic obstructive lung disease
cohort analysis
conference abstract
controlled study
DNA methylation
epigenetics
female
forced vital capacity
fume
gas
genome
human
immune response
linear regression analysis
lung function
major clinical study
male
mineral dust
occupational exposure
promoter region

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1183/1393003.congress-2017.OA2946

Cite this

Van Der Plaat, D., De Vries, M., Bastide-Van Gemert, S., De Jong, K., Van Diemen, C., Nedeljkovic, I., Amin, N., Kromhout, H., Vermeulen, R., Postma, D., Van Duijn, C., Vonk, J., & Boezen, M. (2017). DNA methylation mediates the association between occupational exposures and lung function. European Respiratory Journal, 50(suppl 61), Article OA2946. https://doi.org/10.1183/1393003.congress-2017.OA2946

@article{02d5c0b6cf3240b9a4077c1121aa2b2b,

title = "DNA methylation mediates the association between occupational exposures and lung function",

abstract = "Introduction: Occupational exposures, such as biological dust, mineral dust and gases/fumes, are associated with lower lung function levels and attribute to 15-20% of all Chronic Obstructive Pulmonary Disease (COPD) cases. Epigenetic mechanisms such as DNA methylation have been suggested to play a role in these associations. Aim: To assess if the association between occupational exposures and lung function (FEV /FVC) is mediated by DNA methylation. Methods: We included 1,561 subjects of the LifeLines cohort with either no, low, or high occupational exposure to biological dust, mineral dust and gases/fumes based on the current or last held job. Associations between the three exposures and 420,938 blood DNA methylation sites (CpGs, Illumina 450K array) were assessed using robust linear regression adjusted for appropriate confounders. Differentially methylated regions (DMRs) were identified using comb-p in python. Mediation of the top site per region was assessed using bootstrapping in R. Results: Using p",

keywords = "biological product, endogenous compound, galectin 3, glucocorticoid receptor, adult, bootstrapping, chronic obstructive lung disease, cohort analysis, conference abstract, controlled study, DNA methylation, epigenetics, female, forced vital capacity, fume, gas, genome, human, immune response, linear regression analysis, lung function, major clinical study, male, mineral dust, occupational exposure, promoter region",

author = "{Van Der Plaat}, Diana and {De Vries}, Maaike and {Bastide-Van Gemert}, Sacha and {De Jong}, Kim and {Van Diemen}, Cleo and Ivana Nedeljkovic and Najaf Amin and Hans Kromhout and Roel Vermeulen and Dirkje Postma and {Van Duijn}, Cornelia and Judith Vonk and Marike Boezen",

year = "2017",

month = sep,

day = "1",

doi = "10.1183/1393003.congress-2017.OA2946",

language = "English",

volume = "50",

journal = "European Respiratory Journal",

issn = "0903-1936",

publisher = "European Respiratory Society",

number = "suppl 61",

}

Van Der Plaat, D, De Vries, M, Bastide-Van Gemert, S, De Jong, K, Van Diemen, C, Nedeljkovic, I, Amin, N, Kromhout, H , Vermeulen, R, Postma, D, Van Duijn, C, Vonk, J & Boezen, M 2017, 'DNA methylation mediates the association between occupational exposures and lung function', European Respiratory Journal, vol. 50, no. suppl 61, OA2946. https://doi.org/10.1183/1393003.congress-2017.OA2946

TY - JOUR

T1 - DNA methylation mediates the association between occupational exposures and lung function

AU - Van Der Plaat, Diana

AU - De Vries, Maaike

AU - Bastide-Van Gemert, Sacha

AU - De Jong, Kim

AU - Van Diemen, Cleo

AU - Nedeljkovic, Ivana

AU - Amin, Najaf

AU - Kromhout, Hans

AU - Vermeulen, Roel

AU - Postma, Dirkje

AU - Van Duijn, Cornelia

AU - Vonk, Judith

AU - Boezen, Marike

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Introduction: Occupational exposures, such as biological dust, mineral dust and gases/fumes, are associated with lower lung function levels and attribute to 15-20% of all Chronic Obstructive Pulmonary Disease (COPD) cases. Epigenetic mechanisms such as DNA methylation have been suggested to play a role in these associations. Aim: To assess if the association between occupational exposures and lung function (FEV /FVC) is mediated by DNA methylation. Methods: We included 1,561 subjects of the LifeLines cohort with either no, low, or high occupational exposure to biological dust, mineral dust and gases/fumes based on the current or last held job. Associations between the three exposures and 420,938 blood DNA methylation sites (CpGs, Illumina 450K array) were assessed using robust linear regression adjusted for appropriate confounders. Differentially methylated regions (DMRs) were identified using comb-p in python. Mediation of the top site per region was assessed using bootstrapping in R. Results: Using p

AB - Introduction: Occupational exposures, such as biological dust, mineral dust and gases/fumes, are associated with lower lung function levels and attribute to 15-20% of all Chronic Obstructive Pulmonary Disease (COPD) cases. Epigenetic mechanisms such as DNA methylation have been suggested to play a role in these associations. Aim: To assess if the association between occupational exposures and lung function (FEV /FVC) is mediated by DNA methylation. Methods: We included 1,561 subjects of the LifeLines cohort with either no, low, or high occupational exposure to biological dust, mineral dust and gases/fumes based on the current or last held job. Associations between the three exposures and 420,938 blood DNA methylation sites (CpGs, Illumina 450K array) were assessed using robust linear regression adjusted for appropriate confounders. Differentially methylated regions (DMRs) were identified using comb-p in python. Mediation of the top site per region was assessed using bootstrapping in R. Results: Using p

KW - biological product

KW - endogenous compound

KW - galectin 3

KW - glucocorticoid receptor

KW - adult

KW - bootstrapping

KW - chronic obstructive lung disease

KW - cohort analysis

KW - conference abstract

KW - controlled study

KW - DNA methylation

KW - epigenetics

KW - female

KW - forced vital capacity

KW - fume

KW - gas

KW - genome

KW - human

KW - immune response

KW - linear regression analysis

KW - lung function

KW - major clinical study

KW - male

KW - mineral dust

KW - occupational exposure

KW - promoter region

U2 - 10.1183/1393003.congress-2017.OA2946

DO - 10.1183/1393003.congress-2017.OA2946

M3 - Meeting Abstract

SN - 0903-1936

VL - 50

JO - European Respiratory Journal

JF - European Respiratory Journal

IS - suppl 61

M1 - OA2946