Distribution of zoonotic variegated squirrel bornavirus 1 in naturally infected variegated and Prevost's squirrels

Jana Petzold; Judith M A van den Brand; Daniel Nobach; Bernd Hoffmann; Donata Hoffmann; Christine Fast; Chantal B E M Reusken; Peter R W A van Run; Kore Schlottau; Martin Beer; Christiane Herden

doi:10.1038/s41598-019-47767-4

Distribution of zoonotic variegated squirrel bornavirus 1 in naturally infected variegated and Prevost's squirrels

Jana Petzold, Judith M A van den Brand, Daniel Nobach, Bernd Hoffmann, Donata Hoffmann, Christine Fast, Chantal B E M Reusken, Peter R W A van Run, Kore Schlottau, Martin Beer, Christiane Herden

Institute of Veterinary Pathology, Justus Liebig University, Giessen, Germany. [email protected].
Institute of Veterinary Pathology, Justus Liebig University, Giessen, Germany.
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Greifswald, Germany.
Centre for Health Protection, National Institute for Public Health and the Environment, Bilthoven, The Netherlands.
Erasmus University Medical Center Rotterdam

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Recently, the zoonotic capacity of the newly discovered variegated squirrel bornavirus 1 (VSBV-1) was confirmed in humans with a lethal encephalitis. Transmission to humans occurred by variegated and Prevost's squirrels as presumed reservoir hosts but possible ways of virus shedding and the route of infection still need to be elucidated. Thus, the tissue distribution of VSBV-1 antigen and RNA was investigated in detail via immunohistochemistry (IHC) in six variegated and eight Prevost's squirrels and by in situ hybridisation (ISH) in one Prevost's squirrel, respectively. VSBV-1 antigen and RNA positive cells were most numerous in the nervous system and were also found in nearly all tissues and different cell types indicating a broad organ and cell tropism of VSBV-1. Presence of VSBV-1 in several organs might indicate potential virus shedding via various routes and implies the risk of intra- and interspecies transmission, respectively.

Original language	English
Article number	11402
Journal	Scientific Reports
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41598-019-47767-4
Publication status	Published - 6 Aug 2019

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Petzold, J., van den Brand, J. M. A., Nobach, D., Hoffmann, B., Hoffmann, D., Fast, C., Reusken, C. B. E. M., van Run, P. R. W. A., Schlottau, K., Beer, M., & Herden, C. (2019). Distribution of zoonotic variegated squirrel bornavirus 1 in naturally infected variegated and Prevost's squirrels. Scientific Reports, 9(1), Article 11402. https://doi.org/10.1038/s41598-019-47767-4

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abstract = "Recently, the zoonotic capacity of the newly discovered variegated squirrel bornavirus 1 (VSBV-1) was confirmed in humans with a lethal encephalitis. Transmission to humans occurred by variegated and Prevost's squirrels as presumed reservoir hosts but possible ways of virus shedding and the route of infection still need to be elucidated. Thus, the tissue distribution of VSBV-1 antigen and RNA was investigated in detail via immunohistochemistry (IHC) in six variegated and eight Prevost's squirrels and by in situ hybridisation (ISH) in one Prevost's squirrel, respectively. VSBV-1 antigen and RNA positive cells were most numerous in the nervous system and were also found in nearly all tissues and different cell types indicating a broad organ and cell tropism of VSBV-1. Presence of VSBV-1 in several organs might indicate potential virus shedding via various routes and implies the risk of intra- and interspecies transmission, respectively.",

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AU - Petzold, Jana

AU - van den Brand, Judith M A

AU - Nobach, Daniel

AU - Hoffmann, Bernd

AU - Hoffmann, Donata

AU - Fast, Christine

AU - Reusken, Chantal B E M

AU - van Run, Peter R W A

AU - Schlottau, Kore

AU - Beer, Martin

AU - Herden, Christiane

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AB - Recently, the zoonotic capacity of the newly discovered variegated squirrel bornavirus 1 (VSBV-1) was confirmed in humans with a lethal encephalitis. Transmission to humans occurred by variegated and Prevost's squirrels as presumed reservoir hosts but possible ways of virus shedding and the route of infection still need to be elucidated. Thus, the tissue distribution of VSBV-1 antigen and RNA was investigated in detail via immunohistochemistry (IHC) in six variegated and eight Prevost's squirrels and by in situ hybridisation (ISH) in one Prevost's squirrel, respectively. VSBV-1 antigen and RNA positive cells were most numerous in the nervous system and were also found in nearly all tissues and different cell types indicating a broad organ and cell tropism of VSBV-1. Presence of VSBV-1 in several organs might indicate potential virus shedding via various routes and implies the risk of intra- and interspecies transmission, respectively.

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Distribution of zoonotic variegated squirrel bornavirus 1 in naturally infected variegated and Prevost's squirrels

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