TY - JOUR
T1 - Dissemination of Methicillin-Susceptible CC398 Staphylococcus aureus strains in a rural Greek area
AU - Sarrou, Styliani
AU - Liakopoulos, Apostolos
AU - Chasioti, Markella
AU - Foka, Antigoni
AU - Fthenakis, Georgios
AU - Billinis, Charalampos
AU - Spyrou, Vassiliki
AU - Pantelidi, Kleoniki
AU - Roussaki-Schulze, Angeliki
AU - Lachanas, Vassilios
AU - Makaritsis, Konstantinos
AU - Skoulakis, Charalampos
AU - Daikos, Georgios L
AU - Dalekos, Georgios
AU - Spiliopoulou, Iris
AU - Petinaki, Efthymia
PY - 2015
Y1 - 2015
N2 - A large collection of Staphylococcus aureus including a. 745 clinically significant isolates that were consecutively recovered from human infections during 2012-2013, b. 19 methicillin-susceptible (MSSA), randomly selected between 2006-2011 from our Staphylococcal Collection, c. 16 human colonizing isolates, and d. 10 strains from colonized animals was investigated for the presence and the molecular characteristics of CC398. The study was conducted in Thessaly, a rural region in Greece. The differentiation of livestock-associated clade from the human clade was based on canSNPs combined with the presence of the φ3 bacteriophage and the tetM, scn, sak, and chp genes. Among the 745 isolates, two MRSA (0.8% of total MRSA) and thirteen MSSA (2.65% of total MSSA) were found to belong to CC398, while, between MSSA of our Staphylococcal Collection, one CC398, isolated in 2010, was detected. One human individual, without prior contact with animals, was found to be colonized by a MSSA CC398. No CC398 was identified among the 10 S. aureus isolated from animals. Based on the molecular markers, the 17 CC398 strains were equally placed in the livestock-associated and in the human clades. This is the first report for the dissemination of S. aureus CC398 among humans in Greece.
AB - A large collection of Staphylococcus aureus including a. 745 clinically significant isolates that were consecutively recovered from human infections during 2012-2013, b. 19 methicillin-susceptible (MSSA), randomly selected between 2006-2011 from our Staphylococcal Collection, c. 16 human colonizing isolates, and d. 10 strains from colonized animals was investigated for the presence and the molecular characteristics of CC398. The study was conducted in Thessaly, a rural region in Greece. The differentiation of livestock-associated clade from the human clade was based on canSNPs combined with the presence of the φ3 bacteriophage and the tetM, scn, sak, and chp genes. Among the 745 isolates, two MRSA (0.8% of total MRSA) and thirteen MSSA (2.65% of total MSSA) were found to belong to CC398, while, between MSSA of our Staphylococcal Collection, one CC398, isolated in 2010, was detected. One human individual, without prior contact with animals, was found to be colonized by a MSSA CC398. No CC398 was identified among the 10 S. aureus isolated from animals. Based on the molecular markers, the 17 CC398 strains were equally placed in the livestock-associated and in the human clades. This is the first report for the dissemination of S. aureus CC398 among humans in Greece.
KW - Animals
KW - Anti-Bacterial Agents/pharmacology
KW - Cattle
KW - Drug Resistance, Multiple, Bacterial
KW - Genes, Bacterial
KW - Greece/epidemiology
KW - Humans
KW - Livestock/microbiology
KW - Methicillin/pharmacology
KW - Microbial Sensitivity Tests
KW - Molecular Typing
KW - Rural Population
KW - Sheep
KW - Staphylococcal Infections/diagnosis
KW - Staphylococcus Phages/genetics
KW - Staphylococcus aureus/classification
KW - Swine
U2 - 10.1371/journal.pone.0122761
DO - 10.1371/journal.pone.0122761
M3 - Article
C2 - 25835293
SN - 1932-6203
VL - 10
SP - e0122761
JO - PLoS One
JF - PLoS One
IS - 4
ER -