Differential sensitivity of Rauscher murine leukaemia virus (MuLV-R) to interferons in two interferon responsive cell lines

P. T. Allen, H. Schellekens, L. J.L.D. Van Griensven, A. Billiau

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The effect of interferon on the replication of vesicular stomatitis virus (VSV) and type C oncornavirus in 2 Balb/c mouse bone marrow cell lines, JLS V5 and JLS V9R, infected with MuLV R was examined. VSV replication was inhibited threefold (0.5 log 10) in both cell lines by 10 to 20 units of interferon/ml. In JLS V5 cells C type virus yields, as measured by 3H uridine incorporation and reverse transcriptase activity, were also reduced threefold by 10 to 20 units of interferon/ml. However, in JLS V9R cells, C type virus replication was refractory to interferon at concentrations up to 1x104 units/ml. Infectious C type virus transmitted from JLS V9R cells to Balb/3T3 cells was as sensitive to interferon as virus transmitted from JLS V5 cells, indicating that resistance of C type virus in JLS V9R cells is a feature of the cells rather than of the virus strain.

Original languageEnglish
Pages (from-to)429-435
Number of pages7
JournalJournal of General Virology
Volume31
Issue number3
DOIs
Publication statusPublished - 1 Jan 1976

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