Dietary non-digestible oligosaccharides reduce the sensitizing capacity of deoxynivalenol in a dose-dependent manner

D. Veening-Griffioen, T. Wehkamp, S. Braber, J. Garssen, L. Knippels, P. Jeurink

Research output: Contribution to journalMeeting AbstractAcademic

Abstract

Background: Detrimental compound-exposure in early life can negatively impact normal immune development. Recently, we showed that the mycotoxin deoxynivalenol (DON), a fungal metabolite found in grain-based human diets, can disrupt intestinal tight junctions and can subsequently promote allergic sensitization. Since specific dietary non-digestible oligosaccharides have been shown to protect the gastro-intestinal barrier against DON-induced damage, we hypothesized that these oligosaccharides might also protect against sensitization to cow's milk proteins due to the inhibition of DONinduced gut damage. Method: Upon arrival, female C3H/HeOuJ mice were fed either control cow's milk free AIN93G diet or AIN93G diet supplemented with a 0.5, 1, 2 or 4 w/v% mixture of a 9:1 ratio of short chain galacto-oligosaccharides and long chain fructo-oligosaccharides throughout the study. After 2 weeks, mice were sensitized five times weekly with 5 mg/kg DON with or without 20 mg whey protein. At D35, whey was i.d. injected (10 μg whey/20 ll PBS/ear) in the ear pinnae and the corresponding delta ear swelling at 1 h was measured as readout for the local activation of mast cells. Serum (D37) was collected 30 min after the subsequent oral challenge (50 mg whey/0.5 ml PBS/mouse) to assess the mucosal mast cell response (mMCP-1). At D37, blood and immunological relevant organs were isolated. Results: DON + whey exposure induced an increased acute allergic ear (skin) swelling as compared to DON + PBS exposed animals, both on control diet. The specific oligosaccharide mixture decreased the allergic ear swelling response in a dose-dependent manner as compared to the positive control animals. No alterations in the overall low allergen-specific antibody or mMCP-1 levels were found, whereas there was a trend towards a dose-dependent reduction of serum ST2 (IL-33 receptor) levels in mice on the oligosaccharide-containing diet. Conclusion: Our study suggests that supplementation with dietary non-digestible oligosaccharides can help to protect the epithelial barrier disruption by DON, and to reduce the allergy-inducing capacity of DON.
Original languageEnglish
Pages (from-to)496-497
Number of pages2
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume71
DOIs
Publication statusPublished - 1 Aug 2016
EventEuropean Academy of Allergy and Clinical Immunology Congress - Vienna, Austria
Duration: 11 Jun 201615 Jun 2016

Keywords

  • allergen
  • antibody
  • endogenous compound
  • interleukin 33
  • milk protein
  • oligosaccharide
  • vomitoxin
  • whey protein
  • allergy
  • animal experiment
  • animal model
  • animal tissue
  • blood
  • diet
  • ear
  • exposure
  • female
  • gastrointestinal tract
  • gene inactivation
  • mast cell
  • milk
  • mouse
  • nonhuman
  • organ
  • sensitization
  • skin swelling
  • whey

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