Dietary galacto-oligosaccharides reduce airway eosinophilia and enhance the Th2 suppressive effect of budesonide in house dust mite-induced asthma in mice

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Abstract

Background: In house dust mite (HDM) allergic asthma, symptoms occur due to airway eosinophilia and Th2 cell activation. Budesonide is used to treat airway inflammation and hyper-responsiveness. We showed that dietary non-digestible galacto-oligosaccharides (GOS) suppress symptoms in a murine model for HDM-induced asthma. Method: BALB/c mice were intranasally (i.n.) sensitized with PBS in presence or absence of 1 μg HDM and challenged i.n. with PBS or 10 μg HDM on days 7 till 11 while being fed a diet containing 0, 1 or 2.5 w/w% GOS. On day 7, 9, 11, and 13 budesonide was either or not instilled oropharyngeally. On day 14, airway resistance to metacholine and inflammation were determined. Leukocyte subtypes were analyzed in the broncho-alveolar lavage (BAL) and in lung cell suspensions. Mucosal mast cell protease-1 (mmcp-1) was measured in serum and cytokines in lung homogenates. Results: HDM allergy significantly increased airway responsiveness and BAL leukocyte numbers. Budesonide treatment suppressed this, which reached significance in mice fed GOS. Budesonide reduced the number of lymphocytes and eosinophils in the BAL. Feeding GOS in absence of budesonide treatment reduced the number of eosinophils as well. In addition, both GOS as well as budesonide reduced mmcp- 1 serum concentrations. Interestingly, only in the GOS fed mice, budesonide treatment reduced IL-33 and IL-13 concentrations and the frequency of Th2 cells in the lung. Conclusion: Dietary intervention using GOS may be a novel way to improve effectiveness of anti-inflammatory drug therapy in asthma.
Original languageEnglish
Pages (from-to)44
Number of pages1
JournalAllergy
Volume70
DOIs
Publication statusPublished - 1 Sept 2015

Bibliographical note

Abstracts from the European Academy of Allergy and Clinical Immunology Congress, 6–10 June 2015, Barcelona, Spain

Keywords

  • budesonide
  • galactose oligosaccharide
  • cytokine
  • brinase
  • methacholine
  • interleukin 33
  • antiinflammatory agent
  • airway
  • eosinophilia
  • Dermatophagoides
  • asthma
  • mouse
  • European
  • allergy
  • clinical immunology
  • leukocyte
  • Th2 cell
  • eosinophil
  • murine model
  • lymphocyte
  • respiratory tract inflammation
  • lung homogenate
  • serum
  • cell activation
  • mast cell
  • cell suspension
  • lung alveolus cell
  • lavage
  • allergic asthma
  • drug therapy
  • inflammation
  • airway resistance
  • diet
  • blood level
  • feeding
  • Bagg albino mouse
  • lung

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