Abstract
OBJECTIVE: Long-chain n-3 polyunsaturated fatty acids from oily fish reduce blood pressure (BP) in hypertension. Previously, we demonstrated that hypertension is associated with marked alterations in sphingolipid biology and elevated ceramide-induced vasoconstriction. Here we investigated in spontaneously hypertensive rats (SHRs) whether fish oil improves endothelial function including reduced vascular contraction induced via the sphingolipid cascade, resulting in reduced BP. METHODS: Twelve-week-old SHRs were fed a control or fish oil-enriched diet during 12 weeks, and BP was recorded. Plasma sphingolipid levels were quantified by mass spectrometry and the response of isolated carotid arteries towards different stimuli was measured. Furthermore, erythrocyte membrane fatty acid composition, thromboxane A2formation and cytokine secretion in ex-vivo lipopolysaccharide-stimulated thoracic aorta segments were determined. RESULTS: The fish oil diet reduced the mean arterial BP (P
Original language | English |
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Pages (from-to) | 1050-1058 |
Number of pages | 9 |
Journal | Journal of Hypertension |
Volume | 32 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1 May 2014 |
Keywords
- blood pressure
- ceramide
- endothelial function
- fish oil
- long-chain n-3 polyunsaturated fatty acid
- sphingolipid
- spontaneously hypertensive rat
- arachidonic acid
- docosahexaenoic acid
- glucosylceramide
- icosapentaenoic acid
- interleukin 10
- lipopolysaccharide
- methacholine
- omega 3 fatty acid
- RANTES
- thromboxane
- thromboxane A2
- thromboxane B2
- animal experiment
- animal model
- article
- blood pressure measurement
- blood vessel function
- blood vessel tone
- carotid artery
- cell membrane
- controlled study
- cytokine release
- diet supplementation
- diet therapy
- erythrocyte
- erythrocyte membrane
- essential hypertension
- ex vivo study
- hypertension
- in vivo study
- lipid composition
- male
- mass spectrometry
- nonhuman
- priority journal
- rat
- T lymphocyte
- thoracic aorta
- vasoconstriction