Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing

J.M.A. Brinkhof; D.J. Houwers; L. Moll; D. Dercksen; C. van Maanen

doi:10.1016/j.vetmic.2009.09.060

Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing

J.M.A. Brinkhof^*, D.J. Houwers, L. Moll, D. Dercksen, C. van Maanen

^*Corresponding author for this work

extern

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The objective of the study was to evaluate the diagnostic performances of the ELITEST-MVV ELISA for detection of antibodies against small ruminant lentiviruses and of two recently published PCRs for the detection of proviral DNA of SRLV in blood and corresponding individual milk samples. In addition, the feasibility of bulk milk testing was investigated by titrating ELISA positive pooled milk samples in negative milk, and by investigating bulk milk samples by ELISA and PCR in relation to the SRLV-status of the flocks.

The results show that plasma and milk are suitable replacements for serum. For sheep, both PCRs showed a better diagnostic performance than for goats. ELISA results for bulk milk samples were promising with a putative detection limit of

Original language	English
Pages (from-to)	193-198
Number of pages	6
Journal	Veterinary Microbiology
Volume	142
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetmic.2009.09.060
Publication status	Published - 2010

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10.1016/j.vetmic.2009.09.060

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Brinkhof, J. M. A., Houwers, D. J., Moll, L., Dercksen, D., & van Maanen, C. (2010). Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing. Veterinary Microbiology, 142(3-4), 193-198. https://doi.org/10.1016/j.vetmic.2009.09.060

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title = "Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing",

abstract = "The objective of the study was to evaluate the diagnostic performances of the ELITEST-MVV ELISA for detection of antibodies against small ruminant lentiviruses and of two recently published PCRs for the detection of proviral DNA of SRLV in blood and corresponding individual milk samples. In addition, the feasibility of bulk milk testing was investigated by titrating ELISA positive pooled milk samples in negative milk, and by investigating bulk milk samples by ELISA and PCR in relation to the SRLV-status of the flocks.The results show that plasma and milk are suitable replacements for serum. For sheep, both PCRs showed a better diagnostic performance than for goats. ELISA results for bulk milk samples were promising with a putative detection limit of ",

author = "J.M.A. Brinkhof and D.J. Houwers and L. Moll and D. Dercksen and {van Maanen}, C.",

year = "2010",

doi = "10.1016/j.vetmic.2009.09.060",

language = "English",

volume = "142",

pages = "193--198",

journal = "Veterinary Microbiology",

issn = "0378-1135",

publisher = "Elsevier BV",

number = "3-4",

}

Brinkhof, JMA, Houwers, DJ, Moll, L, Dercksen, D & van Maanen, C 2010, 'Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing', Veterinary Microbiology, vol. 142, no. 3-4, pp. 193-198. https://doi.org/10.1016/j.vetmic.2009.09.060

Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing. / Brinkhof, J.M.A.; Houwers, D.J.; Moll, L. et al.
In: Veterinary Microbiology, Vol. 142, No. 3-4, 2010, p. 193-198.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing

AU - Brinkhof, J.M.A.

AU - Houwers, D.J.

AU - Moll, L.

AU - Dercksen, D.

AU - van Maanen, C.

PY - 2010

Y1 - 2010

N2 - The objective of the study was to evaluate the diagnostic performances of the ELITEST-MVV ELISA for detection of antibodies against small ruminant lentiviruses and of two recently published PCRs for the detection of proviral DNA of SRLV in blood and corresponding individual milk samples. In addition, the feasibility of bulk milk testing was investigated by titrating ELISA positive pooled milk samples in negative milk, and by investigating bulk milk samples by ELISA and PCR in relation to the SRLV-status of the flocks.The results show that plasma and milk are suitable replacements for serum. For sheep, both PCRs showed a better diagnostic performance than for goats. ELISA results for bulk milk samples were promising with a putative detection limit of

AB - The objective of the study was to evaluate the diagnostic performances of the ELITEST-MVV ELISA for detection of antibodies against small ruminant lentiviruses and of two recently published PCRs for the detection of proviral DNA of SRLV in blood and corresponding individual milk samples. In addition, the feasibility of bulk milk testing was investigated by titrating ELISA positive pooled milk samples in negative milk, and by investigating bulk milk samples by ELISA and PCR in relation to the SRLV-status of the flocks.The results show that plasma and milk are suitable replacements for serum. For sheep, both PCRs showed a better diagnostic performance than for goats. ELISA results for bulk milk samples were promising with a putative detection limit of

U2 - 10.1016/j.vetmic.2009.09.060

DO - 10.1016/j.vetmic.2009.09.060

M3 - Article

SN - 0378-1135

VL - 142

SP - 193

EP - 198

JO - Veterinary Microbiology

JF - Veterinary Microbiology

IS - 3-4

ER -

Diagnostic performance of ELISA and PCR in identifying SRLV-infected sheep and goats using serum, plasma and milk samples and in early detection of infection in dairy flocks through bulk milk testing

Abstract

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