Determination of BHV1 specific immune reactivity in naturally infected and vaccinated animals by lymphocyte proliferation assays

V. P.M.G. Rutten; G. H. Wentink; W. A.C. De Jong; A. C.A. Van Exsel; E. J. Hensen

doi:10.1016/0165-2427(90)90049-X

Determination of BHV1 specific immune reactivity in naturally infected and vaccinated animals by lymphocyte proliferation assays

V. P.M.G. Rutten^*, G. H. Wentink, W. A.C. De Jong, A. C.A. Van Exsel, E. J. Hensen

^*Corresponding author for this work

Immunology

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The in vitro BHV1-specific lymphocyte stimulation assay was used to investigate immune reactivity of cattle after natural infection or vaccination with BHV1. Proliferative responses to live virus were shown in tests with peripheral blood lymphocytes of seropositive field virus-infected animals and of vaccinated animals. Nineteen out of 36 seropositive field virus-infected animals did not show in vitro responses. Nine out of 12 animals showed, at least transient, responsiveness after vaccination. Antibody titers were maintained throughout the observation period. T cell activity is believed to play a role in protection against BHV1 infection. The in vitro proliferative assay, however, can not discriminate between BHV1 seropositive and seronegative field virus-infected animals. After vaccination, the BHV1-specific lymphocyte responses of at least one animal disappeared. Both observations may point to the fact that T cell memory is generated, or at least systemically present, to a limited extent.

Original language	English
Pages (from-to)	259-267
Number of pages	9
Journal	Veterinary Immunology and Immunopathology
Volume	25
Issue number	3
DOIs	https://doi.org/10.1016/0165-2427(90)90049-X
Publication status	Published - Jul 1990

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title = "Determination of BHV1 specific immune reactivity in naturally infected and vaccinated animals by lymphocyte proliferation assays",

abstract = "The in vitro BHV1-specific lymphocyte stimulation assay was used to investigate immune reactivity of cattle after natural infection or vaccination with BHV1. Proliferative responses to live virus were shown in tests with peripheral blood lymphocytes of seropositive field virus-infected animals and of vaccinated animals. Nineteen out of 36 seropositive field virus-infected animals did not show in vitro responses. Nine out of 12 animals showed, at least transient, responsiveness after vaccination. Antibody titers were maintained throughout the observation period. T cell activity is believed to play a role in protection against BHV1 infection. The in vitro proliferative assay, however, can not discriminate between BHV1 seropositive and seronegative field virus-infected animals. After vaccination, the BHV1-specific lymphocyte responses of at least one animal disappeared. Both observations may point to the fact that T cell memory is generated, or at least systemically present, to a limited extent.",

author = "Rutten, {V. P.M.G.} and Wentink, {G. H.} and {De Jong}, {W. A.C.} and {Van Exsel}, {A. C.A.} and Hensen, {E. J.}",

year = "1990",

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doi = "10.1016/0165-2427(90)90049-X",

language = "English",

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AU - Rutten, V. P.M.G.

AU - Wentink, G. H.

AU - De Jong, W. A.C.

AU - Van Exsel, A. C.A.

AU - Hensen, E. J.

PY - 1990/7

Y1 - 1990/7

N2 - The in vitro BHV1-specific lymphocyte stimulation assay was used to investigate immune reactivity of cattle after natural infection or vaccination with BHV1. Proliferative responses to live virus were shown in tests with peripheral blood lymphocytes of seropositive field virus-infected animals and of vaccinated animals. Nineteen out of 36 seropositive field virus-infected animals did not show in vitro responses. Nine out of 12 animals showed, at least transient, responsiveness after vaccination. Antibody titers were maintained throughout the observation period. T cell activity is believed to play a role in protection against BHV1 infection. The in vitro proliferative assay, however, can not discriminate between BHV1 seropositive and seronegative field virus-infected animals. After vaccination, the BHV1-specific lymphocyte responses of at least one animal disappeared. Both observations may point to the fact that T cell memory is generated, or at least systemically present, to a limited extent.

AB - The in vitro BHV1-specific lymphocyte stimulation assay was used to investigate immune reactivity of cattle after natural infection or vaccination with BHV1. Proliferative responses to live virus were shown in tests with peripheral blood lymphocytes of seropositive field virus-infected animals and of vaccinated animals. Nineteen out of 36 seropositive field virus-infected animals did not show in vitro responses. Nine out of 12 animals showed, at least transient, responsiveness after vaccination. Antibody titers were maintained throughout the observation period. T cell activity is believed to play a role in protection against BHV1 infection. The in vitro proliferative assay, however, can not discriminate between BHV1 seropositive and seronegative field virus-infected animals. After vaccination, the BHV1-specific lymphocyte responses of at least one animal disappeared. Both observations may point to the fact that T cell memory is generated, or at least systemically present, to a limited extent.

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JO - Veterinary Immunology and Immunopathology

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ER -

Determination of BHV1 specific immune reactivity in naturally infected and vaccinated animals by lymphocyte proliferation assays

Abstract

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