TY - JOUR
T1 - Detection of bovine meat and bone meal in animal feed at a level of 0.1%
AU - Aarts, Henk J M
AU - Bouw, El M.
AU - Buntjer, Jaap B.
AU - Lenstra, Johannes A.
AU - Van Raamsdonk, Leo W D
PY - 2006/11/1
Y1 - 2006/11/1
N2 - For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR® Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133°C for 20 min, a contamination level of 0.1% was detected.
AB - For the control of the transmission of bovine spongiform encephalopathy in cattle via feedstuff, a real-time polymerase chain reaction assay was developed with ruminant-specific Bov-B SINE primers, SYBR® Green fluorescence detection, and melting curve analysis. In formulated cattle and chicken feed samples spiked with pure bovine and sheep meat and bone meal heated at 133°C for 20 min, a contamination level of 0.1% was detected.
UR - http://www.scopus.com/inward/record.url?scp=33845753944&partnerID=8YFLogxK
M3 - Article
C2 - 17225589
AN - SCOPUS:33845753944
SN - 1060-3271
VL - 89
SP - 1443
EP - 1446
JO - Journal of AOAC International
JF - Journal of AOAC International
IS - 6
ER -