Detection and Characterization of Feline Calicivirus Associated with Paw and Mouth Disease

Andrea Palombieri, Vittorio Sarchese, Maria Veronica Giordano, Paola Fruci, Paolo Emidio Crisi, Giovanni Aste, Laura Bongiovanni, Valentina Rinaldi, Alessio Sposato, Michele Camero, Gianvito Lanave, Vito Martella, Fulvio Marsilio, Barbara Di Martino, Federica Di Profio*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Feline calicivirus (FCV) infection in cats can led to several diverse clinical presentations, ranging from mild upper respiratory signs to virulent systemic disease. Herein, we report a paw and mouth disease case in a 7-year-old household cat due to an FCV infection. An asymptomatic cat living in the same household was also infected with FCV. Clinical and pathological investigations were combined with the molecular and phenotypical characterization of the FCV strains. The RNA of the FCV was detected using qualitative and quantitative reverse transcription (RT)-PCR assays, and FCV antigen was confirmed by immunohistochemistry. After the whole genome analysis, the strains detected in the two cats appeared to be genetically diverse from FCVs previously detected in association with paw and mouth disease and with virulent systemic disease. Interestingly, the isolates obtained in this study were resistant to low pH conditions and slightly susceptible to bile salts, but they were susceptible to a trypsin treatment, revealing a phenotype pattern that is different from that which has been observed for respiratory FCVs.

Original languageEnglish
Article number65
Number of pages12
JournalAnimals
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 2023

Bibliographical note

Publisher Copyright:
© 2022 by the authors.

Funding

The present study has been carried out in the framework of the Project “Demetra” (Dipartimenti di Eccellenza 2018–2022, CUP_C46C18000530001), and it was funded by the Italian Ministry for Education, University and Research.

FundersFunder number
Ministero dell’Istruzione, dell’Università e della Ricerca

    Keywords

    • Fcv
    • Immunohistochemistry
    • Paw and mouth disease
    • Phenotypic and antigenic characterization
    • Wgs

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