Detailed analysis of the D-galactose catabolic pathways in Aspergillus niger reveals complexity at both metabolic and regulatory level

Tania Chroumpi; Natalia Martínez-Reyes; Roland S. Kun; Mao Peng; Anna Lipzen; Vivian Ng; Sravanthi Tejomurthula; Yu Zhang; Igor V. Grigoriev; Miia R. Mäkelä; Ronald P. de Vries; Sandra Garrigues

doi:10.1016/j.fgb.2022.103670

Detailed analysis of the D-galactose catabolic pathways in Aspergillus niger reveals complexity at both metabolic and regulatory level

Tania Chroumpi, Natalia Martínez-Reyes, Roland S. Kun, Mao Peng, Anna Lipzen, Vivian Ng, Sravanthi Tejomurthula, Yu Zhang, Igor V. Grigoriev, Miia R. Mäkelä, Ronald P. de Vries^*, Sandra Garrigues

^*Corresponding author for this work

Molecular Plant Physiology

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The current impetus towards a sustainable bio-based economy has accelerated research to better understand the mechanisms through which filamentous fungi convert plant biomass, a valuable feedstock for biotechnological applications. Several transcription factors have been reported to control the polysaccharide degradation and metabolism of the resulting sugars in fungi. However, little is known about their individual contributions, interactions and crosstalk. D-galactose is a hexose sugar present mainly in hemicellulose and pectin in plant biomass. Here, we study D-galactose conversion by Aspergillus niger and describe the involvement of the arabinanolytic and xylanolytic activators AraR and XlnR, in addition to the D-galactose-responsive regulator GalX. Our results deepen the understanding of the complexity of the filamentous fungal regulatory network for plant biomass degradation and sugar catabolism, and facilitate the generation of more efficient plant biomass-degrading strains for biotechnological applications.

Original language	English
Article number	103670
Number of pages	10
Journal	Fungal Genetics and Biology
Volume	159
DOIs	https://doi.org/10.1016/j.fgb.2022.103670
Publication status	Published - Apr 2022

Bibliographical note

Funding Information:
TC was supported by a grant of the NWO ALWOP.233 to RPdV. RSK and SG were supported by a grant of the Applied Science division (TTW) of NWO and the Technology Program of the Ministry of Infrastructure and Water Management 15807 to RPdV. The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The Academy of Finland grant no. 308284 to MRM is also acknowledged.

Publisher Copyright:
© 2022 The Author(s)

Funding

TC was supported by a grant of the NWO ALWOP.233 to RPdV. RSK and SG were supported by a grant of the Applied Science division (TTW) of NWO and the Technology Program of the Ministry of Infrastructure and Water Management 15807 to RPdV. The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The Academy of Finland grant no. 308284 to MRM is also acknowledged.

Keywords

Aspergillus niger
D-galactose catabolism
Leloir pathway
Oxido-reductive D-galactose catabolic pathway
Pentose Catabolic Pathway (PCP)
Transcription factors

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Chroumpi, T., Martínez-Reyes, N., Kun, R. S., Peng, M., Lipzen, A., Ng, V., Tejomurthula, S., Zhang, Y., Grigoriev, I. V., Mäkelä, M. R., de Vries, R. P., & Garrigues, S. (2022). Detailed analysis of the D-galactose catabolic pathways in Aspergillus niger reveals complexity at both metabolic and regulatory level. Fungal Genetics and Biology, 159, Article 103670. https://doi.org/10.1016/j.fgb.2022.103670

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abstract = "The current impetus towards a sustainable bio-based economy has accelerated research to better understand the mechanisms through which filamentous fungi convert plant biomass, a valuable feedstock for biotechnological applications. Several transcription factors have been reported to control the polysaccharide degradation and metabolism of the resulting sugars in fungi. However, little is known about their individual contributions, interactions and crosstalk. D-galactose is a hexose sugar present mainly in hemicellulose and pectin in plant biomass. Here, we study D-galactose conversion by Aspergillus niger and describe the involvement of the arabinanolytic and xylanolytic activators AraR and XlnR, in addition to the D-galactose-responsive regulator GalX. Our results deepen the understanding of the complexity of the filamentous fungal regulatory network for plant biomass degradation and sugar catabolism, and facilitate the generation of more efficient plant biomass-degrading strains for biotechnological applications.",

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note = "Funding Information: TC was supported by a grant of the NWO ALWOP.233 to RPdV. RSK and SG were supported by a grant of the Applied Science division (TTW) of NWO and the Technology Program of the Ministry of Infrastructure and Water Management 15807 to RPdV. The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The Academy of Finland grant no. 308284 to MRM is also acknowledged. Publisher Copyright: {\textcopyright} 2022 The Author(s)",

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Chroumpi, T, Martínez-Reyes, N, Kun, RS, Peng, M, Lipzen, A, Ng, V, Tejomurthula, S, Zhang, Y, Grigoriev, IV, Mäkelä, MR, de Vries, RP & Garrigues, S 2022, 'Detailed analysis of the D-galactose catabolic pathways in Aspergillus niger reveals complexity at both metabolic and regulatory level', Fungal Genetics and Biology, vol. 159, 103670. https://doi.org/10.1016/j.fgb.2022.103670

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AU - Chroumpi, Tania

AU - Martínez-Reyes, Natalia

AU - Kun, Roland S.

AU - Peng, Mao

AU - Lipzen, Anna

AU - Ng, Vivian

AU - Tejomurthula, Sravanthi

AU - Zhang, Yu

AU - Grigoriev, Igor V.

AU - Mäkelä, Miia R.

AU - de Vries, Ronald P.

AU - Garrigues, Sandra

N1 - Funding Information: TC was supported by a grant of the NWO ALWOP.233 to RPdV. RSK and SG were supported by a grant of the Applied Science division (TTW) of NWO and the Technology Program of the Ministry of Infrastructure and Water Management 15807 to RPdV. The work conducted by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The Academy of Finland grant no. 308284 to MRM is also acknowledged. Publisher Copyright: © 2022 The Author(s)

PY - 2022/4

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N2 - The current impetus towards a sustainable bio-based economy has accelerated research to better understand the mechanisms through which filamentous fungi convert plant biomass, a valuable feedstock for biotechnological applications. Several transcription factors have been reported to control the polysaccharide degradation and metabolism of the resulting sugars in fungi. However, little is known about their individual contributions, interactions and crosstalk. D-galactose is a hexose sugar present mainly in hemicellulose and pectin in plant biomass. Here, we study D-galactose conversion by Aspergillus niger and describe the involvement of the arabinanolytic and xylanolytic activators AraR and XlnR, in addition to the D-galactose-responsive regulator GalX. Our results deepen the understanding of the complexity of the filamentous fungal regulatory network for plant biomass degradation and sugar catabolism, and facilitate the generation of more efficient plant biomass-degrading strains for biotechnological applications.

AB - The current impetus towards a sustainable bio-based economy has accelerated research to better understand the mechanisms through which filamentous fungi convert plant biomass, a valuable feedstock for biotechnological applications. Several transcription factors have been reported to control the polysaccharide degradation and metabolism of the resulting sugars in fungi. However, little is known about their individual contributions, interactions and crosstalk. D-galactose is a hexose sugar present mainly in hemicellulose and pectin in plant biomass. Here, we study D-galactose conversion by Aspergillus niger and describe the involvement of the arabinanolytic and xylanolytic activators AraR and XlnR, in addition to the D-galactose-responsive regulator GalX. Our results deepen the understanding of the complexity of the filamentous fungal regulatory network for plant biomass degradation and sugar catabolism, and facilitate the generation of more efficient plant biomass-degrading strains for biotechnological applications.

KW - Aspergillus niger

KW - D-galactose catabolism

KW - Leloir pathway

KW - Oxido-reductive D-galactose catabolic pathway

KW - Pentose Catabolic Pathway (PCP)

KW - Transcription factors

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C2 - 35121171

AN - SCOPUS:85124025333

SN - 1087-1845

VL - 159

JO - Fungal Genetics and Biology

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M1 - 103670

ER -

Detailed analysis of the D-galactose catabolic pathways in Aspergillus niger reveals complexity at both metabolic and regulatory level

Abstract

Bibliographical note

Funding

Keywords

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