Design and synthesis of reagents for phage display screening of dehalogenases

Roland J. Pieters; Marko Fennema; Richard M. Kellogg; Dick B. Janssen

doi:10.1016/S0960-894X(98)00697-0

Design and synthesis of reagents for phage display screening of dehalogenases

Roland J. Pieters, Marko Fennema, Richard M. Kellogg, Dick B. Janssen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Bifunctional molecules containing both a biotin and a substrate unit have been designed and synthesized for phage display screening of mutant libraries of haloalkane enzymes. The molecules were assembled using a convergent modular synthetic strategy. One molecule was synthesized to evaluate the concept of covalent capture and a second for screening of phage libraries for enantioselectivity.

Original language	English
Pages (from-to)	161-166
Number of pages	6
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	9
Issue number	2
DOIs	https://doi.org/10.1016/S0960-894X(98)00697-0
Publication status	Published - 18 Jan 1999
Externally published	Yes

Keywords

alkane
biotin
mutant protein
streptavidin
article
catalysis
catalyst
chemical structure
chirality
enzyme activity
enzyme substrate
gene mutation
phage display

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10.1016/S0960-894X(98)00697-0

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abstract = "Bifunctional molecules containing both a biotin and a substrate unit have been designed and synthesized for phage display screening of mutant libraries of haloalkane enzymes. The molecules were assembled using a convergent modular synthetic strategy. One molecule was synthesized to evaluate the concept of covalent capture and a second for screening of phage libraries for enantioselectivity.",

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AU - Fennema, Marko

AU - Kellogg, Richard M.

AU - Janssen, Dick B.

PY - 1999/1/18

Y1 - 1999/1/18

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AB - Bifunctional molecules containing both a biotin and a substrate unit have been designed and synthesized for phage display screening of mutant libraries of haloalkane enzymes. The molecules were assembled using a convergent modular synthetic strategy. One molecule was synthesized to evaluate the concept of covalent capture and a second for screening of phage libraries for enantioselectivity.

KW - alkane

KW - biotin

KW - mutant protein

KW - streptavidin

KW - article

KW - catalysis

KW - catalyst

KW - chemical structure

KW - chirality

KW - enzyme activity

KW - enzyme substrate

KW - gene mutation

KW - phage display

U2 - 10.1016/S0960-894X(98)00697-0

DO - 10.1016/S0960-894X(98)00697-0

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EP - 166

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 2

ER -

Design and synthesis of reagents for phage display screening of dehalogenases

Abstract

Keywords

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