Deoxynivalenol exposure during pregnancy has adverse effects on placental structure and immunity in mice model

Negisa Seyed Toutounchi; Saskia Braber; Belinda van‘t Land; Suzan Thijssen; Johan Garssen; Gert Folkerts; Astrid Hogenkamp

doi:10.1016/j.reprotox.2022.07.002

Deoxynivalenol exposure during pregnancy has adverse effects on placental structure and immunity in mice model

Negisa Seyed Toutounchi
, Saskia Braber
, Belinda van‘t Land
, Suzan Thijssen
, Johan Garssen
, Gert Folkerts
, Astrid Hogenkamp^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Deoxynivalenol (DON), a highly prevalent food contaminant, is known to induce reproductive and immunotoxicity in humans upon exposure. The present study focused on the consequences of exposure to DON during pregnancy for placental barrier and immune function, as well as fetal survival. Female mice received diets contaminated with DON (6.25 and 12.5 mg/kg of diet), starting immediately after mating until the end of the experiment. On day 17 of pregnancy the animals were killed, and maternal and fetal samples were collected for further analysis. Feeding on DON-contaminated diets decreased fetal survival, and DON was detected at significant levels in the fetus. Placentae from DON-exposed mice revealed a reduction in expression of junctional proteins, ZO-1, E-cadherin and claudins, upregulation of AHR mRNA expressions, and increase in IFN-ꝩ, IL-6 and IL-4 production. In conclusion, results of this study demonstrate harmful effects of DON on the course of pregnancy and fetal survival, which might be due to immunological changes in maternal immune organs and placenta. Altogether, these data underline the importance of the quality of maternal diet during pregnancy as they clearly demonstrate the potential harmful effects of a commonly present food-contaminant.

Original language	English
Pages (from-to)	109-118
Number of pages	10
Journal	Reproductive Toxicology
Volume	112
DOIs	https://doi.org/10.1016/j.reprotox.2022.07.002
Publication status	Published - Sept 2022

Bibliographical note

Funding Information:
The authors would like to thank Prescilla Jeurink for her helpful contribution to discussions.

Publisher Copyright:
© 2022

Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

Funding

The authors would like to thank Prescilla Jeurink for her helpful contribution to discussions.

Keywords

Deoxynivalenol
Mycotoxin
Placenta
Pregnancy
Reproductive toxicity

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Cite this

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title = "Deoxynivalenol exposure during pregnancy has adverse effects on placental structure and immunity in mice model",

abstract = "Deoxynivalenol (DON), a highly prevalent food contaminant, is known to induce reproductive and immunotoxicity in humans upon exposure. The present study focused on the consequences of exposure to DON during pregnancy for placental barrier and immune function, as well as fetal survival. Female mice received diets contaminated with DON (6.25 and 12.5 mg/kg of diet), starting immediately after mating until the end of the experiment. On day 17 of pregnancy the animals were killed, and maternal and fetal samples were collected for further analysis. Feeding on DON-contaminated diets decreased fetal survival, and DON was detected at significant levels in the fetus. Placentae from DON-exposed mice revealed a reduction in expression of junctional proteins, ZO-1, E-cadherin and claudins, upregulation of AHR mRNA expressions, and increase in IFN-ꝩ, IL-6 and IL-4 production. In conclusion, results of this study demonstrate harmful effects of DON on the course of pregnancy and fetal survival, which might be due to immunological changes in maternal immune organs and placenta. Altogether, these data underline the importance of the quality of maternal diet during pregnancy as they clearly demonstrate the potential harmful effects of a commonly present food-contaminant.",

keywords = "Deoxynivalenol, Mycotoxin, Placenta, Pregnancy, Reproductive toxicity",

author = "Toutounchi, \{Negisa Seyed\} and Saskia Braber and Land, \{Belinda van{\textquoteleft}t\} and Suzan Thijssen and Johan Garssen and Gert Folkerts and Astrid Hogenkamp",

note = "Funding Information: The authors would like to thank Prescilla Jeurink for her helpful contribution to discussions. Publisher Copyright: {\textcopyright} 2022 Copyright {\textcopyright} 2022 The Authors. Published by Elsevier Inc. All rights reserved.",

year = "2022",

month = sep,

doi = "10.1016/j.reprotox.2022.07.002",

language = "English",

volume = "112",

pages = "109--118",

journal = "Reproductive Toxicology",

issn = "0890-6238",

publisher = "Elsevier Inc.",

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T1 - Deoxynivalenol exposure during pregnancy has adverse effects on placental structure and immunity in mice model

AU - Toutounchi, Negisa Seyed

AU - Braber, Saskia

AU - Land, Belinda van‘t

AU - Thijssen, Suzan

AU - Garssen, Johan

AU - Folkerts, Gert

AU - Hogenkamp, Astrid

N1 - Funding Information: The authors would like to thank Prescilla Jeurink for her helpful contribution to discussions. Publisher Copyright: © 2022 Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2022/9

Y1 - 2022/9

N2 - Deoxynivalenol (DON), a highly prevalent food contaminant, is known to induce reproductive and immunotoxicity in humans upon exposure. The present study focused on the consequences of exposure to DON during pregnancy for placental barrier and immune function, as well as fetal survival. Female mice received diets contaminated with DON (6.25 and 12.5 mg/kg of diet), starting immediately after mating until the end of the experiment. On day 17 of pregnancy the animals were killed, and maternal and fetal samples were collected for further analysis. Feeding on DON-contaminated diets decreased fetal survival, and DON was detected at significant levels in the fetus. Placentae from DON-exposed mice revealed a reduction in expression of junctional proteins, ZO-1, E-cadherin and claudins, upregulation of AHR mRNA expressions, and increase in IFN-ꝩ, IL-6 and IL-4 production. In conclusion, results of this study demonstrate harmful effects of DON on the course of pregnancy and fetal survival, which might be due to immunological changes in maternal immune organs and placenta. Altogether, these data underline the importance of the quality of maternal diet during pregnancy as they clearly demonstrate the potential harmful effects of a commonly present food-contaminant.

AB - Deoxynivalenol (DON), a highly prevalent food contaminant, is known to induce reproductive and immunotoxicity in humans upon exposure. The present study focused on the consequences of exposure to DON during pregnancy for placental barrier and immune function, as well as fetal survival. Female mice received diets contaminated with DON (6.25 and 12.5 mg/kg of diet), starting immediately after mating until the end of the experiment. On day 17 of pregnancy the animals were killed, and maternal and fetal samples were collected for further analysis. Feeding on DON-contaminated diets decreased fetal survival, and DON was detected at significant levels in the fetus. Placentae from DON-exposed mice revealed a reduction in expression of junctional proteins, ZO-1, E-cadherin and claudins, upregulation of AHR mRNA expressions, and increase in IFN-ꝩ, IL-6 and IL-4 production. In conclusion, results of this study demonstrate harmful effects of DON on the course of pregnancy and fetal survival, which might be due to immunological changes in maternal immune organs and placenta. Altogether, these data underline the importance of the quality of maternal diet during pregnancy as they clearly demonstrate the potential harmful effects of a commonly present food-contaminant.

KW - Deoxynivalenol

KW - Mycotoxin

KW - Placenta

KW - Pregnancy

KW - Reproductive toxicity

UR - https://www.scopus.com/pages/publications/85134628463

U2 - 10.1016/j.reprotox.2022.07.002

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SN - 0890-6238

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JO - Reproductive Toxicology

JF - Reproductive Toxicology

ER -

Deoxynivalenol exposure during pregnancy has adverse effects on placental structure and immunity in mice model

Abstract

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Keywords

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