Deciphering the Proteome Dynamics during Development of Neurons Derived from Induced Pluripotent Stem Cells

Suzy Varderidou-Minasian, Bert M. Verheijen, Philipp Schätzle, Casper C. Hoogenraad, R. Jeroen Pasterkamp, Maarten Altelaar

Research output: Contribution to journalArticleAcademicpeer-review


Neuronal development is a complex multistep process that shapes neurons by progressing though several typical stages, including axon outgrowth, dendrite formation, and synaptogenesis. Knowledge of the mechanisms of neuronal development is mostly derived from the study of animal models. Advances in stem cell technology now enable us to generate neurons from human induced pluripotent stem cells (iPSCs). Here we provide a mass spectrometry-based quantitative proteomic signature of human iPSC-derived neurons, i.e., iPSC-derived induced glutamatergic neurons and iPSC-derived motor neurons, throughout neuronal differentiation. Tandem mass tag 10-plex labeling was carried out to perform proteomic profiling of cells at different time points. Our analysis reveals significant expression changes (FDR < 0.001) of several key proteins during the differentiation process, e.g., proteins involved in the Wnt and Notch signaling pathways. Overall, our data provide a rich resource of information on protein expression during human iPSC neuron differentiation.

Original languageEnglish
Pages (from-to)2391-2403
Number of pages13
JournalJournal of Proteome Research
Issue number 6
Publication statusPublished - 5 Jun 2020


  • human development
  • iPSC
  • mass spectrometry
  • neuronal development
  • neuronal differentiation
  • neuroproteomics
  • proteome
  • quantitative proteomics
  • tandem mass tags
  • TMT 10-plex


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