DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells

Martin Harterink; Marta Santos Esteves da Silva; Lena Will; Julia Turan; Adiljan Ibrahim; Alexander E Lang; Eljo Y Van Battum; R Jeroen Pasterkamp; Lukas C Kapitein; Dmitri Kudryashov; Ben A Barres; Casper C Hoogenraad; J Bradley Zuchero

doi:10.1038/nmeth.4257

DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells

Martin Harterink, Marta Santos Esteves da Silva, Lena Will, Julia Turan, Adiljan Ibrahim, Alexander E Lang, Eljo Y Van Battum, R Jeroen Pasterkamp, Lukas C Kapitein, Dmitri Kudryashov, Ben A Barres, Casper C Hoogenraad, J Bradley Zuchero

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.

Original language	English
Pages (from-to)	479-482
Number of pages	4
Journal	Nature Methods
Volume	14
Issue number	5
DOIs	https://doi.org/10.1038/nmeth.4257
Publication status	Published - 2017

Keywords

cell culture
cell migration
cellular neuroscience
cytoskeleton
neurogenesis

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10.1038/nmeth.4257

deactsFinal published version, 3.3 MBLicence: Taverne

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Harterink, M., Santos Esteves da Silva, M., Will, L., Turan, J., Ibrahim, A., Lang, A. E., Van Battum, E. Y., Pasterkamp, R. J., Kapitein, L. C., Kudryashov, D., Barres, B. A., Hoogenraad, C. C., & Zuchero, J. B. (2017). DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells. Nature Methods, 14(5), 479-482. https://doi.org/10.1038/nmeth.4257

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author = "Martin Harterink and {Santos Esteves da Silva}, Marta and Lena Will and Julia Turan and Adiljan Ibrahim and Lang, {Alexander E} and {Van Battum}, {Eljo Y} and Pasterkamp, {R Jeroen} and Kapitein, {Lukas C} and Dmitri Kudryashov and Barres, {Ben A} and Hoogenraad, {Casper C} and Zuchero, {J Bradley}",

year = "2017",

doi = "10.1038/nmeth.4257",

language = "English",

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AU - Harterink, Martin

AU - Santos Esteves da Silva, Marta

AU - Will, Lena

AU - Turan, Julia

AU - Ibrahim, Adiljan

AU - Lang, Alexander E

AU - Van Battum, Eljo Y

AU - Pasterkamp, R Jeroen

AU - Kapitein, Lukas C

AU - Kudryashov, Dmitri

AU - Barres, Ben A

AU - Hoogenraad, Casper C

AU - Zuchero, J Bradley

PY - 2017

Y1 - 2017

N2 - The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.

AB - The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.

KW - cell culture

KW - cell migration

KW - cellular neuroscience

KW - cytoskeleton

KW - neurogenesis

U2 - 10.1038/nmeth.4257

DO - 10.1038/nmeth.4257

M3 - Article

C2 - 28394337

SN - 1548-7091

VL - 14

SP - 479

EP - 482

JO - Nature Methods

JF - Nature Methods

IS - 5

ER -

DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells

Abstract

Keywords

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