Dark-field light scattering microscope with focus stabilization

Anna Peters; Zhu Zhang; Sanli Faez

doi:10.1016/j.ohx.2023.e00424

Dark-field light scattering microscope with focus stabilization

Anna Peters^*, Zhu Zhang, Sanli Faez

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We present detailed design and operation instructions for a single-objective inverted microscope. Our design is suitable for two dark-field modes of operation: 1- total internal reflection scattering, and 2- cross-polarization backscattering. The user can switch between the two modes by exchanging one mode-steering element, which is also adapted to the Thorlabs cage system. To establish a stable background speckle for differential microscopy the imaging plane is stabilized with active feedback. We validate the stabilization efficacy by performing long-term scattering measurement on single nanoparticles. This setup can be extended for simultaneous scattering, fluorescence, and confocal imaging modes.

Original language	English
Article number	e00424
Journal	HardwareX
Volume	14
DOIs	https://doi.org/10.1016/j.ohx.2023.e00424
Publication status	Published - 19 May 2023

Bibliographical note

Publisher Copyright:
© 2023 The Author(s)

Funding

We thank Paul Jurrius, JanBonne Aans, and Dante Killian for technical support. This project was supported by the Dutch Organisation for Scientific Research (NWO) with grant project: Photonics Translational Research – Medical Photonics (MEDPHOT).

Funders	Funder number
Nederlandse Organisatie voor Wetenschappelijk Onderzoek

Keywords

Cross-polarization microscopy
Dark-field imaging
Focus stabilization
Inverted microscope

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10.1016/j.ohx.2023.e00424Licence: CC BY

PIIS2468067223000317Final published version, 1.86 MBLicence: CC BY

Cite this

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abstract = "We present detailed design and operation instructions for a single-objective inverted microscope. Our design is suitable for two dark-field modes of operation: 1- total internal reflection scattering, and 2- cross-polarization backscattering. The user can switch between the two modes by exchanging one mode-steering element, which is also adapted to the Thorlabs cage system. To establish a stable background speckle for differential microscopy the imaging plane is stabilized with active feedback. We validate the stabilization efficacy by performing long-term scattering measurement on single nanoparticles. This setup can be extended for simultaneous scattering, fluorescence, and confocal imaging modes.",

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AU - Faez, Sanli

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AB - We present detailed design and operation instructions for a single-objective inverted microscope. Our design is suitable for two dark-field modes of operation: 1- total internal reflection scattering, and 2- cross-polarization backscattering. The user can switch between the two modes by exchanging one mode-steering element, which is also adapted to the Thorlabs cage system. To establish a stable background speckle for differential microscopy the imaging plane is stabilized with active feedback. We validate the stabilization efficacy by performing long-term scattering measurement on single nanoparticles. This setup can be extended for simultaneous scattering, fluorescence, and confocal imaging modes.

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SN - 2468-0672

VL - 14

JO - HardwareX

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ER -

Dark-field light scattering microscope with focus stabilization

Abstract

Bibliographical note

Funding

Keywords

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