Abstract
BackgroundCyclooxygenase-2 (COX-2) is a key enzyme in the synthesis of pro-inflammatory prostaglandins and 5-lipoxygenase (5-LO) is the major source of leukotrienes. Their role in IBD has been demonstrated in humans and animal models, but not in dogs with chronic enteropathies (CCE).
HypothesisCOX-2 and 5-LO are upregulated in dogs with CCE.
AnimalsFifteen healthy control dogs (HCD), 10 dogs with inflammatory bowel disease (IBD), and 15 dogs with food-responsive diarrhea (FRD).
MethodsProspective study. mRNA expression of COX-2, 5-LO, IL-1b, IL-4, IL-6, TNF, IL-10 and TFG- was evaluated by quantitative real-time RT-PCR in duodenal and colonic biopsies before and after treatment.
ResultsCOX-2 expression in the colon was significantly higher in IBD and FRD before and after treatment (all P
Conclusions and Clinical ImportanceCOX-2 is upregulated in IBD and FRD, whereas IL-1b and TGF- seem to be important pro- and anti-inflammatory cytokines, respectively. The use of dual COX/5-LO inhibitors could be an interesting alternative in the treatment of CCE.
| Original language | English |
|---|---|
| Pages (from-to) | 1684-1691 |
| Number of pages | 8 |
| Journal | Journal of Veterinary Internal Medicine |
| Volume | 28 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2014 |
Funding
The authors thank the Swiss Armed Forces Veterinary Services for their financial support of S. Dumusc. E. C. Ontsouka and C. Albrecht were supported by the Swiss National Science Foundation through the National Centre of Competence in Research TransCure. The expert laboratory work of Mrs. Claudine Morel, Veterinary Physiology, Vetsuisse Faculty University of Bern, is gratefully acknowledged.
Keywords
- Food-responsive diarrhea
- Inflammatory bowel disease
- Interleukin
- Leukotriene
- INFLAMMATORY-BOWEL-DISEASE
- MESSENGER-RNA EXPRESSION
- DUODENAL MUCOSA
- CROHNS-DISEASE
- RT-PCR
- COLITIS
- INHIBITORS
- QUANTIFICATION
- PATHWAY
- CELLS
