Coordination of peptidoglycan synthesis and outer membrane constriction during Escherichia coli cell division

Andrew N Gray, Alexander J F Egan, Inge van 't Veer, Jolanda Verheul, Alexandre Colavin, Alexandra Koumoutsi, Jacob Biboy, A F Maarten Altelaar, Mirjam J Damen, Kerwyn Casey Huang, Jean-Pierre Simorre, Eefjan Breukink, Tanneke den Blaauwen, Athanasios Typas, Carol A Gross, Waldemar Vollmer

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Abstract

To maintain cellular structure and integrity during division, Gram-negative bacteria must carefully coordinate constriction of a tripartite cell envelope of inner membrane, peptidoglycan (PG), and outer membrane (OM). It has remained enigmatic how this is accomplished. Here, we show that envelope machines facilitating septal PG synthesis (PBP1B-LpoB complex) and OM constriction (Tol system) are physically and functionally coordinated via YbgF, renamed CpoB (Coordinator of PG synthesis and OM constriction, associated with PBP1B). CpoB localizes to the septum concurrent with PBP1B-LpoB and Tol at the onset of constriction, interacts with both complexes, and regulates PBP1B activity in response to Tol energy state. This coordination links PG synthesis with OM invagination and imparts a unique mode of bifunctional PG synthase regulation by selectively modulating PBP1B cross-linking activity. Coordination of the PBP1B and Tol machines by CpoB contributes to effective PBP1B function in vivo and maintenance of cell envelope integrity during division.

Original languageEnglish
JournaleLife
Volume4
DOIs
Publication statusPublished - 7 May 2015

Keywords

  • PENICILLIN-BINDING PROTEINS
  • BETA-LACTAM ANTIBIOTICS
  • N-TERMINAL DOMAIN
  • MUREIN-LIPOPROTEIN
  • BACILLUS-SUBTILIS
  • CYTOKINETIC RING
  • WALL HYDROLYSIS
  • INNER MEMBRANE
  • SYNTHASE PBP1B
  • INDUCED LYSIS

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