Cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic States in Arabidopsis

Tuncay Baubec; Huy Q Dinh; Ales Pecinka; Branislava Rakic; Wilfried Rozhon; Bonnie Wohlrab; Arndt von Haeseler; Ortrun Mittelsten Scheid

doi:10.1105/tpc.109.072819

Cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic States in Arabidopsis

Tuncay Baubec, Huy Q Dinh, Ales Pecinka, Branislava Rakic, Wilfried Rozhon, Bonnie Wohlrab, Arndt von Haeseler, Ortrun Mittelsten Scheid

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Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Epigenetic changes of gene expression can potentially be reversed by developmental programs, genetic manipulation, or pharmacological interference. However, a case of transcriptional gene silencing, originally observed in tetraploid Arabidopsis thaliana plants, created an epiallele resistant to many mutations or inhibitor treatments that activate many other suppressed genes. This raised the question about the molecular basis of this extreme stability. A combination of forward and reverse genetics and drug application provides evidence for an epigenetic double lock that is only alleviated upon the simultaneous removal of both DNA methylation and histone methylation. Therefore, the cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic states and contributes to heritable diversity of gene expression patterns.

Original language	English
Pages (from-to)	34-47
Number of pages	14
Journal	The Plant Cell
Volume	22
Issue number	1
DOIs	https://doi.org/10.1105/tpc.109.072819
Publication status	Published - Jan 2010
Externally published	Yes

Keywords

Adenosylhomocysteinase/genetics
Alleles
Arabidopsis/genetics
Arabidopsis Proteins/genetics
Chromatin/metabolism
DNA Methylation
DNA, Bacterial/genetics
DNA, Plant/metabolism
DNA-Binding Proteins/genetics
Epigenesis, Genetic
Gene Expression Regulation, Plant
Histone Deacetylase Inhibitors/metabolism
Histones/metabolism
Mutagenesis, Insertional
Mutation
Polyploidy
Sequence Analysis, DNA
Transcription Factors/genetics
Transcription, Genetic

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10.1105/tpc.109.072819

Cite this

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title = "Cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic States in Arabidopsis",

abstract = "Epigenetic changes of gene expression can potentially be reversed by developmental programs, genetic manipulation, or pharmacological interference. However, a case of transcriptional gene silencing, originally observed in tetraploid Arabidopsis thaliana plants, created an epiallele resistant to many mutations or inhibitor treatments that activate many other suppressed genes. This raised the question about the molecular basis of this extreme stability. A combination of forward and reverse genetics and drug application provides evidence for an epigenetic double lock that is only alleviated upon the simultaneous removal of both DNA methylation and histone methylation. Therefore, the cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic states and contributes to heritable diversity of gene expression patterns.",

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author = "Tuncay Baubec and Dinh, {Huy Q} and Ales Pecinka and Branislava Rakic and Wilfried Rozhon and Bonnie Wohlrab and {von Haeseler}, Arndt and {Mittelsten Scheid}, Ortrun",

year = "2010",

month = jan,

doi = "10.1105/tpc.109.072819",

language = "English",

volume = "22",

pages = "34--47",

journal = "The Plant Cell",

issn = "1040-4651",

publisher = "American Society of Plant Biologists",

number = "1",

}

TY - JOUR

T1 - Cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic States in Arabidopsis

AU - Baubec, Tuncay

AU - Dinh, Huy Q

AU - Pecinka, Ales

AU - Rakic, Branislava

AU - Rozhon, Wilfried

AU - Wohlrab, Bonnie

AU - von Haeseler, Arndt

AU - Mittelsten Scheid, Ortrun

PY - 2010/1

Y1 - 2010/1

N2 - Epigenetic changes of gene expression can potentially be reversed by developmental programs, genetic manipulation, or pharmacological interference. However, a case of transcriptional gene silencing, originally observed in tetraploid Arabidopsis thaliana plants, created an epiallele resistant to many mutations or inhibitor treatments that activate many other suppressed genes. This raised the question about the molecular basis of this extreme stability. A combination of forward and reverse genetics and drug application provides evidence for an epigenetic double lock that is only alleviated upon the simultaneous removal of both DNA methylation and histone methylation. Therefore, the cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic states and contributes to heritable diversity of gene expression patterns.

AB - Epigenetic changes of gene expression can potentially be reversed by developmental programs, genetic manipulation, or pharmacological interference. However, a case of transcriptional gene silencing, originally observed in tetraploid Arabidopsis thaliana plants, created an epiallele resistant to many mutations or inhibitor treatments that activate many other suppressed genes. This raised the question about the molecular basis of this extreme stability. A combination of forward and reverse genetics and drug application provides evidence for an epigenetic double lock that is only alleviated upon the simultaneous removal of both DNA methylation and histone methylation. Therefore, the cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic states and contributes to heritable diversity of gene expression patterns.

KW - Adenosylhomocysteinase/genetics

KW - Alleles

KW - Arabidopsis/genetics

KW - Arabidopsis Proteins/genetics

KW - Chromatin/metabolism

KW - DNA Methylation

KW - DNA, Bacterial/genetics

KW - DNA, Plant/metabolism

KW - DNA-Binding Proteins/genetics

KW - Epigenesis, Genetic

KW - Gene Expression Regulation, Plant

KW - Histone Deacetylase Inhibitors/metabolism

KW - Histones/metabolism

KW - Mutagenesis, Insertional

KW - Mutation

KW - Polyploidy

KW - Sequence Analysis, DNA

KW - Transcription Factors/genetics

KW - Transcription, Genetic

U2 - 10.1105/tpc.109.072819

DO - 10.1105/tpc.109.072819

M3 - Article

C2 - 20097869

SN - 1040-4651

VL - 22

SP - 34

EP - 47

JO - The Plant Cell

JF - The Plant Cell

IS - 1

ER -

Cooperation of multiple chromatin modifications can generate unanticipated stability of epigenetic States in Arabidopsis

Abstract

Keywords

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