Conversion of 11-ketoandrostenedione to 11-ketotestosterone by blood cells of six fish species

I. Mayer; B. Borg; R. Schulz

doi:10.1016/0016-6480(90)90207-3

Conversion of 11-ketoandrostenedione to 11-ketotestosterone by blood cells of six fish species

I. Mayer, B. Borg, R. Schulz

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Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Blood cells from Baltic salmon, Salmo salar, three-spined stickleback, Gasterosteus aculeatus, eel pout, Zoarces viviparus, crucian carp, Carassius carassius, African catfish, Clarias gariepinus, and reedfish, Calamoichthys calabaricus, were incubated with tritiated 11β-hydroxyandrostenedione (OHA) or 11-ketoandrostenedione (OA). In all fish there was conversion of OA to 11-ketotestosterone (OT), indicating that 17β-hydroxysteroid dehydrogenase activity was present in the blood cells. On the other hand, OHA was not converted to 11β-hydroxytestosterone in any fish. The addition of serum to the incubates largely prevented the OA-OT conversion by salmon blood cells.

Original language	English
Pages (from-to)	70-74
Number of pages	5
Journal	General and Comparative Endocrinology
Volume	77
Issue number	1
DOIs	https://doi.org/10.1016/0016-6480(90)90207-3
Publication status	Published - 10 Dec 1990
Externally published	Yes

Keywords

11 oxotestosterone
adrenosterone
radioisotope
animal cell
article
blood cell
cell culture
female
fish
male
metabolism
nonhuman
priority journal

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10.1016/0016-6480(90)90207-3

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title = "Conversion of 11-ketoandrostenedione to 11-ketotestosterone by blood cells of six fish species",

abstract = "Blood cells from Baltic salmon, Salmo salar, three-spined stickleback, Gasterosteus aculeatus, eel pout, Zoarces viviparus, crucian carp, Carassius carassius, African catfish, Clarias gariepinus, and reedfish, Calamoichthys calabaricus, were incubated with tritiated 11β-hydroxyandrostenedione (OHA) or 11-ketoandrostenedione (OA). In all fish there was conversion of OA to 11-ketotestosterone (OT), indicating that 17β-hydroxysteroid dehydrogenase activity was present in the blood cells. On the other hand, OHA was not converted to 11β-hydroxytestosterone in any fish. The addition of serum to the incubates largely prevented the OA-OT conversion by salmon blood cells.",

keywords = "11 oxotestosterone, adrenosterone, radioisotope, animal cell, article, blood cell, cell culture, female, fish, male, metabolism, nonhuman, priority journal",

author = "I. Mayer and B. Borg and R. Schulz",

year = "1990",

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doi = "10.1016/0016-6480(90)90207-3",

language = "English",

volume = "77",

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TY - JOUR

T1 - Conversion of 11-ketoandrostenedione to 11-ketotestosterone by blood cells of six fish species

AU - Mayer, I.

AU - Borg, B.

AU - Schulz, R.

PY - 1990/12/10

Y1 - 1990/12/10

N2 - Blood cells from Baltic salmon, Salmo salar, three-spined stickleback, Gasterosteus aculeatus, eel pout, Zoarces viviparus, crucian carp, Carassius carassius, African catfish, Clarias gariepinus, and reedfish, Calamoichthys calabaricus, were incubated with tritiated 11β-hydroxyandrostenedione (OHA) or 11-ketoandrostenedione (OA). In all fish there was conversion of OA to 11-ketotestosterone (OT), indicating that 17β-hydroxysteroid dehydrogenase activity was present in the blood cells. On the other hand, OHA was not converted to 11β-hydroxytestosterone in any fish. The addition of serum to the incubates largely prevented the OA-OT conversion by salmon blood cells.

AB - Blood cells from Baltic salmon, Salmo salar, three-spined stickleback, Gasterosteus aculeatus, eel pout, Zoarces viviparus, crucian carp, Carassius carassius, African catfish, Clarias gariepinus, and reedfish, Calamoichthys calabaricus, were incubated with tritiated 11β-hydroxyandrostenedione (OHA) or 11-ketoandrostenedione (OA). In all fish there was conversion of OA to 11-ketotestosterone (OT), indicating that 17β-hydroxysteroid dehydrogenase activity was present in the blood cells. On the other hand, OHA was not converted to 11β-hydroxytestosterone in any fish. The addition of serum to the incubates largely prevented the OA-OT conversion by salmon blood cells.

KW - 11 oxotestosterone

KW - adrenosterone

KW - radioisotope

KW - animal cell

KW - article

KW - blood cell

KW - cell culture

KW - female

KW - fish

KW - male

KW - metabolism

KW - nonhuman

KW - priority journal

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DO - 10.1016/0016-6480(90)90207-3

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JO - General and Comparative Endocrinology

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IS - 1

ER -

Conversion of 11-ketoandrostenedione to 11-ketotestosterone by blood cells of six fish species

Abstract

Keywords

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