Convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms

Yuankun Yang*, Christina E Steidele, Clemens Rössner, Birgit Löffelhardt, Dagmar Kolb, Thomas Leisen, Weiguo Zhang, Christina Ludwig, Georg Felix, Michael F Seidl, Annette Becker, Thorsten Nürnberger, Matthias Hahn, Bertolt Gust, Harald Gross, Ralph Hückelhoven, Andrea A Gust*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The Arabidopsis thaliana Receptor-Like Protein RLP30 contributes to immunity against the fungal pathogen Sclerotinia sclerotiorum. Here we identify the RLP30-ligand as a small cysteine-rich protein (SCP) that occurs in many fungi and oomycetes and is also recognized by the Nicotiana benthamiana RLP RE02. However, RLP30 and RE02 share little sequence similarity and respond to different parts of the native/folded protein. Moreover, some Brassicaceae other than Arabidopsis also respond to a linear SCP peptide instead of the folded protein, suggesting that SCP is an eminent immune target that led to the convergent evolution of distinct immune receptors in plants. Surprisingly, RLP30 shows a second ligand specificity for a SCP-nonhomologous protein secreted by bacterial Pseudomonads. RLP30 expression in N. tabacum results in quantitatively lower susceptibility to bacterial, fungal and oomycete pathogens, thus demonstrating that detection of immunogenic patterns by Arabidopsis RLP30 is involved in defense against pathogens from three microbial kingdoms.

Original languageEnglish
Article number3621
Pages (from-to)1-12
Number of pages12
JournalNature Communications
Volume14
Issue number1
DOIs
Publication statusPublished - 19 Jun 2023

Keywords

  • Arabidopsis/metabolism
  • Cysteine/metabolism
  • Ligands
  • Proteins/metabolism
  • Oomycetes/metabolism
  • Bacteria/metabolism
  • Receptors, Pattern Recognition/metabolism
  • Plant Diseases/microbiology
  • Plant Immunity
  • Arabidopsis Proteins/genetics
  • Gene Expression Regulation, Plant

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