Controlled Chemoenzymatic Synthesis of Heparan Sulfate Oligosaccharides

Weigang Lu; Chengli Zong; Pradeep Chopra; Lauren Pepi; Yongmei Xu; Jian Liu; I. Jonathan Amster; Geert-Jan Boons

doi:10.1002/anie.201800387

Controlled Chemoenzymatic Synthesis of Heparan Sulfate Oligosaccharides

Weigang Lu
, Chengli Zong
, Pradeep Chopra
, Lauren Pepi
, Yongmei Xu
, Jian Liu
, I. Jonathan Amster
, Geert-Jan Boons^*

^*Corresponding author for this work

United States [email protected]

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A chemoenzymatic approach has been developed for the preparation of diverse libraries of heparan sulfate (HS) oligosaccharides. It employs chemically synthesized oligosaccharides having a chemical entity at a GlcN residue, which in unanticipated manners influences the site of modification of NST, C5-Epi/2-OST and 6-OST1/6-OST3 resulting in oligosaccharides differing in N/O-sulfation and epimerization pattern. The enzymatic transformations defined fine substrate requirements of NST, C5-Epi, 2-OST and 6-OST.

Original language	English
Pages (from-to)	5340-5344
Number of pages	5
Journal	Angewandte Chemie-International Edition
Volume	57
Issue number	19
DOIs	https://doi.org/10.1002/anie.201800387
Publication status	Published - May 2018

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Journal Article

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10.1002/anie.201800387

Angew Chem Int Ed - 2018 - Lu - Controlled Chemoenzymatic Synthesis of Heparan Sulfate OligosaccharidesFinal published version, 3.36 MBLicence: Taverne

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abstract = "A chemoenzymatic approach has been developed for the preparation of diverse libraries of heparan sulfate (HS) oligosaccharides. It employs chemically synthesized oligosaccharides having a chemical entity at a GlcN residue, which in unanticipated manners influences the site of modification of NST, C5-Epi/2-OST and 6-OST1/6-OST3 resulting in oligosaccharides differing in N/O-sulfation and epimerization pattern. The enzymatic transformations defined fine substrate requirements of NST, C5-Epi, 2-OST and 6-OST.",

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AU - Lu, Weigang

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AU - Pepi, Lauren

AU - Xu, Yongmei

AU - Liu, Jian

AU - Amster, I. Jonathan

AU - Boons, Geert-Jan

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AB - A chemoenzymatic approach has been developed for the preparation of diverse libraries of heparan sulfate (HS) oligosaccharides. It employs chemically synthesized oligosaccharides having a chemical entity at a GlcN residue, which in unanticipated manners influences the site of modification of NST, C5-Epi/2-OST and 6-OST1/6-OST3 resulting in oligosaccharides differing in N/O-sulfation and epimerization pattern. The enzymatic transformations defined fine substrate requirements of NST, C5-Epi, 2-OST and 6-OST.

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JF - Angewandte Chemie-International Edition

IS - 19

ER -

Controlled Chemoenzymatic Synthesis of Heparan Sulfate Oligosaccharides

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