Abstract
A chemoenzymatic approach has been developed for the preparation of diverse libraries of heparan sulfate (HS) oligosaccharides. It employs chemically synthesized oligosaccharides having a chemical entity at a GlcN residue, which in unanticipated manners influences the site of modification of NST, C5-Epi/2-OST and 6-OST1/6-OST3 resulting in oligosaccharides differing in N/O-sulfation and epimerization pattern. The enzymatic transformations defined fine substrate requirements of NST, C5-Epi, 2-OST and 6-OST.
Original language | English |
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Pages (from-to) | 5340-5344 |
Number of pages | 5 |
Journal | Angewandte Chemie-International Edition |
Volume | 57 |
Issue number | 19 |
DOIs | |
Publication status | Published - May 2018 |
Keywords
- Journal Article