Controlled Chemoenzymatic Synthesis of Heparan Sulfate Oligosaccharides

Weigang Lu, Chengli Zong, Pradeep Chopra, Lauren Pepi, Yongmei Xu, Jian Liu, I. Jonathan Amster, Geert-Jan Boons

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

A chemoenzymatic approach has been developed for the preparation of diverse libraries of heparan sulfate (HS) oligosaccharides. It employs chemically synthesized oligosaccharides having a chemical entity at a GlcN residue, which in unanticipated manners influences the site of modification of NST, C5-Epi/2-OST and 6-OST1/6-OST3 resulting in oligosaccharides differing in N/O-sulfation and epimerization pattern. The enzymatic transformations defined fine substrate requirements of NST, C5-Epi, 2-OST and 6-OST.

Original languageEnglish
Pages (from-to)5340-5344
Number of pages5
JournalAngewandte Chemie-International Edition
Volume57
Issue number19
DOIs
Publication statusPublished - May 2018

Keywords

  • Journal Article

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