Complement 5a Receptor deficiency does not influence adverse cardiac remodeling after pressure-overload in mice

Judith J. De Haan, Lena Bosch, Anouska Borgman, Marissa Bastemeijer, Maike A.D. Brans, Sander M. Van De Weg, Dominique P.V. De Kleijn, Joost P.G. Sluijter, Hamid El Azzouzi, Saskia C.A. De Jager*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Hypertension is one of the most common risk factors for the development heart failure in the general population. Inflammation plays a central role in this adverse remodeling and eventually to the development of heart failure. Circulating levels of Complement factor 5a (C5a) are increased in hypertensive patients and the C5a receptor is associated with the presence of cardiac fibrosis and inflammation in an experimental hypertension model. To test if C5aR is involved in adverse cardiac remodeling following pressure-overload, we induced transverse aortic constriction (TAC) in wildtype and C5a receptor deficient mice (C5aR-/-). Six weeks after TAC, C5aR-/- animals showed a similar degree of cardiac hypertrophy and decrease in cardiac function as wild type mice (End Systolic Volume; 50.30±5.32 μl vs. 55.81±8.16 μl). In addition, other features of adverse cardiac remodeling like cardiomyocyte cell size (WGA staining), fibrosis (picrosirius red staining) or collagen degradation (matrix metalloproteinase activity assay) did not differ either. In conclusion, full body C5aR deficiency does not affect adverse cardiac remodeling after pressure-overload. However, our finding are in contrast with C5a inhibition studies. Our observations do present the role of C5a-C5aR in adverse cardiac remodeling and heart failure as controversial at the least.

Original languageEnglish
Article number17045
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017
Externally publishedYes

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