Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals

Jaap A. Wagenaar; M.A.P. van Bergen; D G Newell; R Grogono-Thomas; B Duim

doi:10.1128/JCM.39.6.2283-2286.2001

Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals

Jaap A. Wagenaar
, M.A.P. van Bergen
, D G Newell
, R Grogono-Thomas
, B Duim

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A collection of Campylobacter fetus strains, including both C. fetus subsp. fetus and C. fetus subsp. venerealis, were phenotypically identified to the subspecies level and genotypically typed by PCR and amplified fragment length polymorphism (AFLP) analysis. Phenotypic subspecies determination methods were unreliable. Genotyping of the strains by PCR and AFLP showed a clear discrimination between the two subspecies.

Original language	English
Pages (from-to)	2283-2286
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	39
Issue number	6
DOIs	https://doi.org/10.1128/JCM.39.6.2283-2286.2001
Publication status	Published - Jun 2001

Keywords

Animals
Bacterial Typing Techniques
Campylobacter fetus
DNA Fingerprinting
Genotype
Phenotype
Polymerase Chain Reaction
Polymorphism, Genetic

Access to Document

10.1128/JCM.39.6.2283-2286.2001

Cite this

Wagenaar, J. A., van Bergen, M. A. P., Newell, D. G., Grogono-Thomas, R., & Duim, B. (2001). Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals. Journal of Clinical Microbiology, 39(6), 2283-2286. https://doi.org/10.1128/JCM.39.6.2283-2286.2001

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title = "Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals",

abstract = "A collection of Campylobacter fetus strains, including both C. fetus subsp. fetus and C. fetus subsp. venerealis, were phenotypically identified to the subspecies level and genotypically typed by PCR and amplified fragment length polymorphism (AFLP) analysis. Phenotypic subspecies determination methods were unreliable. Genotyping of the strains by PCR and AFLP showed a clear discrimination between the two subspecies.",

keywords = "Animals, Bacterial Typing Techniques, Campylobacter fetus, DNA Fingerprinting, Genotype, Phenotype, Polymerase Chain Reaction, Polymorphism, Genetic",

author = "Wagenaar, \{Jaap A.\} and \{van Bergen\}, M.A.P. and Newell, \{D G\} and R Grogono-Thomas and B Duim",

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Wagenaar, JA, van Bergen, MAP, Newell, DG, Grogono-Thomas, R & Duim, B 2001, 'Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals', Journal of Clinical Microbiology, vol. 39, no. 6, pp. 2283-2286. https://doi.org/10.1128/JCM.39.6.2283-2286.2001

Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals. / Wagenaar, Jaap A.; van Bergen, M.A.P.; Newell, D G et al.
In: Journal of Clinical Microbiology, Vol. 39, No. 6, 06.2001, p. 2283-2286.

Research output: Contribution to journal › Article › Academic › peer-review

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AU - Grogono-Thomas, R

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KW - Bacterial Typing Techniques

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KW - DNA Fingerprinting

KW - Genotype

KW - Phenotype

KW - Polymerase Chain Reaction

KW - Polymorphism, Genetic

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