Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals

Jaap A. Wagenaar, M.A.P. van Bergen, D G Newell, R Grogono-Thomas, B Duim

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    A collection of Campylobacter fetus strains, including both C. fetus subsp. fetus and C. fetus subsp. venerealis, were phenotypically identified to the subspecies level and genotypically typed by PCR and amplified fragment length polymorphism (AFLP) analysis. Phenotypic subspecies determination methods were unreliable. Genotyping of the strains by PCR and AFLP showed a clear discrimination between the two subspecies.

    Original languageEnglish
    Pages (from-to)2283-2286
    Number of pages4
    JournalJournal of Clinical Microbiology
    Volume39
    Issue number6
    DOIs
    Publication statusPublished - Jun 2001

    Keywords

    • Animals
    • Bacterial Typing Techniques
    • Campylobacter fetus
    • DNA Fingerprinting
    • Genotype
    • Phenotype
    • Polymerase Chain Reaction
    • Polymorphism, Genetic

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