Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model

Barry Rockx; Thijs Kuiken; Sander Herfst; Theo Bestebroer; Mart M. Lamers; Bas B. Oude Munnink; Dennis de Meulder; Geert van Amerongen; Judith van den Brand; Nisreen M. A. Okba; Debby Schipper; Peter van Run; Lonneke Leijten; Reina Sikkema; Ernst Verschoor; Babs Verstrepen; Willy Bogers; Jan Langermans; Christian Drosten; Martje Fentener van Vlissingen; Ron Fouchier; Rik de Swart; Marion Koopmans; Bart L. Haagmans

doi:10.1126/science.abb7314

Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model

Barry Rockx, Thijs Kuiken, Sander Herfst, Theo Bestebroer, Mart M. Lamers, Bas B. Oude Munnink, Dennis de Meulder, Geert van Amerongen, Judith van den Brand, Nisreen M. A. Okba, Debby Schipper, Peter van Run, Lonneke Leijten, Reina Sikkema, Ernst Verschoor, Babs Verstrepen, Willy Bogers, Jan Langermans, Christian Drosten, Martje Fentener van VlissingenRon Fouchier, Rik de Swart, Marion Koopmans, Bart L. Haagmans

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The current pandemic coronavirus, severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2), was
recently identified in patients with an acute respiratory syndrome, coronavirus disease 2019 (COVID-19).
To compare its pathogenesis with that of previously emerging coronaviruses, we inoculated cynomolgus
macaques with SARS-CoV-2 or Middle East respiratory syndrome (MERS)–CoV and compared the
pathology and virology with historical reports of SARS-CoV infections. In SARS-CoV-2–infected
macaques, virus was excreted from nose and throat in the absence of clinical signs and detected in
type I and II pneumocytes in foci of diffuse alveolar damage and in ciliated epithelial cells of nasal,
bronchial, and bronchiolar mucosae. In SARS-CoV infection, lung lesions were typically more severe,
whereas they were milder in MERS-CoV infection, where virus was detected mainly in type II
pneumocytes. These data show that SARS-CoV-2 causes COVID-19–like disease in macaques and
provides a new model to test preventive and therapeutic strategies.

Original language	English
Pages (from-to)	1012-1015
Journal	Science
Volume	368
Issue number	6494
DOIs	https://doi.org/10.1126/science.abb7314
Publication status	Published - 29 May 2020

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Rockx, B., Kuiken, T., Herfst, S., Bestebroer, T., Lamers, M. M., Munnink, B. B. O., de Meulder, D., van Amerongen, G., van den Brand, J., Okba, N. M. A., Schipper, D., van Run, P., Leijten, L., Sikkema, R., Verschoor, E., Verstrepen, B., Bogers, W., Langermans, J., Drosten, C., ... Haagmans, B. L. (2020). Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model. Science, 368(6494), 1012-1015. https://doi.org/10.1126/science.abb7314

@article{83ffc784d6c44e799a1b824c7995b8ed,

title = "Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model",

abstract = "The current pandemic coronavirus, severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2), wasrecently identified in patients with an acute respiratory syndrome, coronavirus disease 2019 (COVID-19).To compare its pathogenesis with that of previously emerging coronaviruses, we inoculated cynomolgusmacaques with SARS-CoV-2 or Middle East respiratory syndrome (MERS)–CoV and compared thepathology and virology with historical reports of SARS-CoV infections. In SARS-CoV-2–infectedmacaques, virus was excreted from nose and throat in the absence of clinical signs and detected intype I and II pneumocytes in foci of diffuse alveolar damage and in ciliated epithelial cells of nasal,bronchial, and bronchiolar mucosae. In SARS-CoV infection, lung lesions were typically more severe,whereas they were milder in MERS-CoV infection, where virus was detected mainly in type IIpneumocytes. These data show that SARS-CoV-2 causes COVID-19–like disease in macaques andprovides a new model to test preventive and therapeutic strategies.",

author = "Barry Rockx and Thijs Kuiken and Sander Herfst and Theo Bestebroer and Lamers, {Mart M.} and Munnink, {Bas B. Oude} and {de Meulder}, Dennis and {van Amerongen}, Geert and {van den Brand}, Judith and Okba, {Nisreen M. A.} and Debby Schipper and {van Run}, Peter and Lonneke Leijten and Reina Sikkema and Ernst Verschoor and Babs Verstrepen and Willy Bogers and Jan Langermans and Christian Drosten and {van Vlissingen}, {Martje Fentener} and Ron Fouchier and {de Swart}, Rik and Marion Koopmans and Haagmans, {Bart L.}",

year = "2020",

month = may,

day = "29",

doi = "10.1126/science.abb7314",

language = "English",

volume = "368",

pages = "1012--1015",

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Rockx, B, Kuiken, T, Herfst, S, Bestebroer, T, Lamers, MM, Munnink, BBO, de Meulder, D, van Amerongen, G, van den Brand, J, Okba, NMA, Schipper, D, van Run, P, Leijten, L, Sikkema, R, Verschoor, E, Verstrepen, B, Bogers, W, Langermans, J, Drosten, C, van Vlissingen, MF, Fouchier, R, de Swart, R, Koopmans, M & Haagmans, BL 2020, 'Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model', Science, vol. 368, no. 6494, pp. 1012-1015. https://doi.org/10.1126/science.abb7314

TY - JOUR

T1 - Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model

AU - Rockx, Barry

AU - Kuiken, Thijs

AU - Herfst, Sander

AU - Bestebroer, Theo

AU - Lamers, Mart M.

AU - Munnink, Bas B. Oude

AU - de Meulder, Dennis

AU - van Amerongen, Geert

AU - van den Brand, Judith

AU - Okba, Nisreen M. A.

AU - Schipper, Debby

AU - van Run, Peter

AU - Leijten, Lonneke

AU - Sikkema, Reina

AU - Verschoor, Ernst

AU - Verstrepen, Babs

AU - Bogers, Willy

AU - Langermans, Jan

AU - Drosten, Christian

AU - van Vlissingen, Martje Fentener

AU - Fouchier, Ron

AU - de Swart, Rik

AU - Koopmans, Marion

AU - Haagmans, Bart L.

PY - 2020/5/29

Y1 - 2020/5/29

N2 - The current pandemic coronavirus, severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2), wasrecently identified in patients with an acute respiratory syndrome, coronavirus disease 2019 (COVID-19).To compare its pathogenesis with that of previously emerging coronaviruses, we inoculated cynomolgusmacaques with SARS-CoV-2 or Middle East respiratory syndrome (MERS)–CoV and compared thepathology and virology with historical reports of SARS-CoV infections. In SARS-CoV-2–infectedmacaques, virus was excreted from nose and throat in the absence of clinical signs and detected intype I and II pneumocytes in foci of diffuse alveolar damage and in ciliated epithelial cells of nasal,bronchial, and bronchiolar mucosae. In SARS-CoV infection, lung lesions were typically more severe,whereas they were milder in MERS-CoV infection, where virus was detected mainly in type IIpneumocytes. These data show that SARS-CoV-2 causes COVID-19–like disease in macaques andprovides a new model to test preventive and therapeutic strategies.

AB - The current pandemic coronavirus, severe acute respiratory syndrome–coronavirus 2 (SARS-CoV-2), wasrecently identified in patients with an acute respiratory syndrome, coronavirus disease 2019 (COVID-19).To compare its pathogenesis with that of previously emerging coronaviruses, we inoculated cynomolgusmacaques with SARS-CoV-2 or Middle East respiratory syndrome (MERS)–CoV and compared thepathology and virology with historical reports of SARS-CoV infections. In SARS-CoV-2–infectedmacaques, virus was excreted from nose and throat in the absence of clinical signs and detected intype I and II pneumocytes in foci of diffuse alveolar damage and in ciliated epithelial cells of nasal,bronchial, and bronchiolar mucosae. In SARS-CoV infection, lung lesions were typically more severe,whereas they were milder in MERS-CoV infection, where virus was detected mainly in type IIpneumocytes. These data show that SARS-CoV-2 causes COVID-19–like disease in macaques andprovides a new model to test preventive and therapeutic strategies.

U2 - 10.1126/science.abb7314

DO - 10.1126/science.abb7314

M3 - Article

SN - 0036-8075

VL - 368

SP - 1012

EP - 1015

JO - Science

JF - Science

IS - 6494

ER -

Comparative pathogenesis of COVID-19, MERS, and SARS in a nonhuman primate model

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