COLLECTION AND COMPOSITION OF SALIVA FROM SUCKLING PIGLETS

Eva de Goede, F. Bikker, T.J. Tobias

    Research output: Contribution to conferenceAbstractOther research output

    Abstract

    Background and objectives
    The potential composition of saliva and the easy way in which it can be collected, makes it an important matrix for the diagnosis of several diseases or biomarkers. Therefore, the aim of this study was twofold: to develop best
    practices for saliva collection of individual suckling piglets and determine age- related protein patterns in saliva.

    Material and methods
    A randomized block design was used with 5 groups of 32 suckling piglets from 18 litters of different ages (1-2 days, 1, 2, 3 and 4 weeks old) which had to chew for 0.5, 1, 2 and 4 minutes on a Salivette for saliva collection. The quantity, protein concentration, protease activity and protein composition in the saliva samples were determined with a protein assay, protease assay and SDS-page.

    Results
    It was found that a minimal sampling time of 2 minutes is necessary to collect sufficient saliva in individual suckling piglets from ~2 weeks and older to perform the abovementioned analyses. In contrast, in younger piglets, saliva collection was hardly possible, even at 4 min collection time. Both amount of saliva collected and protein concentration increased with sampling time and both parameters were dependent on age. Protease activity was associated with saliva quantity, protein concentration and a random litter effect. Moreover, the
    protease activity was higher in piglets from 3 and 4 weeks old than in younger piglets. Finally, the longer the chewing time, the higher the probability that proteins with a mass of 110 and 20 kDa were detected in saliva samples. So far the identity of these proteins has not been revealed.

    Conclusion
    To obtain sufficient saliva from each piglet, individual saliva sampling is possible in pigs ?2 weeks old and requires 2 minutes of chewing time per piglet. With these samples various biochemical analysis can be performed.
    Original languageEnglish
    Pages432
    Publication statusPublished - 22 May 2019
    Event11th European Symposium of Porcine Health Management (ESPHM) - TivoliVredenburg, Utrecht, Netherlands
    Duration: 22 May 201924 May 2019
    http://www.esphm2019.org

    Conference

    Conference11th European Symposium of Porcine Health Management (ESPHM)
    Abbreviated titleESPHM2019
    Country/TerritoryNetherlands
    CityUtrecht
    Period22/05/1924/05/19
    Internet address

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