Cloning and characterization of the 5'-flanking region of the canine growth hormone gene

Irma S Lantinga-van Leeuwen, Elpetra A P Timmermans-Sprang, Jan A Mol

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The growth hormone (GH) gene is expressed in a variety of tissues outside the pituitary, including the mammary gland. GH expression in the mammary gland is stimulated by progestins. The local synthesis of mammary GH may provide a highly proliferative environment within the mammary gland that may contribute to the development or progression of mammary tumours. To elucidate the mechanism regulating mammary GH expression, we cloned the 5'-flanking region of the canine GH gene using inverse polymerase chain reaction. Gel-shift experiments showed that several sequences in the 5'-flanking region of the GH gene bind mammary nuclear proteins and may be involved in basal and progesterone-induced mammary GH expression. Sequence analysis and comparison with the GH promoters of human, rat, and mouse genes revealed a number of shared binding sites for transcription factors such as Pit-1, which is involved in pituitary GH expression, and for factors involved in the differentiation of lymphoid cells. Moreover, a putative binding site for the progesterone receptor (PR) was identified in all promoters, indicating that the progestin-induced expression of GH in mammary tissue is most probably a direct effect of activated PRs on the GH gene promoter and that this may occur in various species.

Original languageEnglish
Pages (from-to)133-41
Number of pages9
JournalMolecular and Cellular Endocrinology
Volume197
Issue number1-2
DOIs
Publication statusPublished - 29 Nov 2002

Keywords

  • 5' Flanking Region
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • Dogs/genetics
  • Growth Hormone/chemistry
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Protein Binding
  • Transcription Factors/metabolism

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