Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori

Amin Talebi Bezmin Abadi; Ashraf Mohhabati Mobarez; Marc J M Bonten; Jaap A Wagenaar; Johannes G Kusters

doi:10.1186/1471-230X-14-33

Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori

Amin Talebi Bezmin Abadi, Ashraf Mohhabati Mobarez, Marc J M Bonten, Jaap A Wagenaar, Johannes G Kusters

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Numerous proteins have been proposed as virulence factors for the gram negative gastric bacterium Helicobacter pylori but only for a few this has unequivocally been demonstrated. The aim of the current study was to evaluate the association of the putative virulence factors tnpA and tnpB (no cagA) with H. pylori associated gastroduodenal diseases.

METHODS: A PCR based assay was used to determine the presence of the tnpA and tnpB genes, as well as of cagA, in 360H. pylori strains isolated from H. pylori infected patients.

RESULTS: Of 360 H. pylori culture positive patients (196 men, 164 women; average age 42.1 years (range 17-73), 95 had gastritis, 92 had gastric ulcers, 108 had duodenal ulcers, and 65 had gastric cancer. Using the gastritis group as a reference a significantly aberrant gene distribution was observed for the tnpA (Relative risk: 1.45; 95% CI 1.04-1.93), the cagA (Relative risk: 1.81; 95% CI 1.44-2.29), but not the tnpB gene in the gastric cancer group.

CONCLUSIONS: The increased incidence of the tnpA gene in gastric cancer patients suggests a role of the tnpA gene in the development of H. pylori induced gastric cancer.

Original language	English
Article number	14:33
Journal	BMC Gastroenterology
Volume	14
DOIs	https://doi.org/10.1186/1471-230X-14-33
Publication status	Published - 2014

Keywords

Adolescent
Adult
Aged
Antigens, Bacterial
Bacterial Proteins
Cross-Sectional Studies
DNA, Bacterial
Duodenal Ulcer
Female
Gastritis
Helicobacter Infections
Helicobacter pylori
Humans
Iran
Male
Middle Aged
Stomach Neoplasms
Stomach Ulcer
Virulence Factors
Young Adult

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori",

abstract = "BACKGROUND: Numerous proteins have been proposed as virulence factors for the gram negative gastric bacterium Helicobacter pylori but only for a few this has unequivocally been demonstrated. The aim of the current study was to evaluate the association of the putative virulence factors tnpA and tnpB (no cagA) with H. pylori associated gastroduodenal diseases.METHODS: A PCR based assay was used to determine the presence of the tnpA and tnpB genes, as well as of cagA, in 360H. pylori strains isolated from H. pylori infected patients.RESULTS: Of 360 H. pylori culture positive patients (196 men, 164 women; average age 42.1 years (range 17-73), 95 had gastritis, 92 had gastric ulcers, 108 had duodenal ulcers, and 65 had gastric cancer. Using the gastritis group as a reference a significantly aberrant gene distribution was observed for the tnpA (Relative risk: 1.45; 95% CI 1.04-1.93), the cagA (Relative risk: 1.81; 95% CI 1.44-2.29), but not the tnpB gene in the gastric cancer group.CONCLUSIONS: The increased incidence of the tnpA gene in gastric cancer patients suggests a role of the tnpA gene in the development of H. pylori induced gastric cancer.",

keywords = "Adolescent, Adult, Aged, Antigens, Bacterial, Bacterial Proteins, Cross-Sectional Studies, DNA, Bacterial, Duodenal Ulcer, Female, Gastritis, Helicobacter Infections, Helicobacter pylori, Humans, Iran, Male, Middle Aged, Stomach Neoplasms, Stomach Ulcer, Virulence Factors, Young Adult",

author = "Abadi, {Amin Talebi Bezmin} and Mobarez, {Ashraf Mohhabati} and Bonten, {Marc J M} and Wagenaar, {Jaap A} and Kusters, {Johannes G}",

year = "2014",

doi = "10.1186/1471-230X-14-33",

language = "English",

volume = "14",

journal = "BMC Gastroenterology",

issn = "1471-230X",

publisher = "BioMed Central",

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T1 - Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori

AU - Abadi, Amin Talebi Bezmin

AU - Mobarez, Ashraf Mohhabati

AU - Bonten, Marc J M

AU - Wagenaar, Jaap A

AU - Kusters, Johannes G

PY - 2014

Y1 - 2014

N2 - BACKGROUND: Numerous proteins have been proposed as virulence factors for the gram negative gastric bacterium Helicobacter pylori but only for a few this has unequivocally been demonstrated. The aim of the current study was to evaluate the association of the putative virulence factors tnpA and tnpB (no cagA) with H. pylori associated gastroduodenal diseases.METHODS: A PCR based assay was used to determine the presence of the tnpA and tnpB genes, as well as of cagA, in 360H. pylori strains isolated from H. pylori infected patients.RESULTS: Of 360 H. pylori culture positive patients (196 men, 164 women; average age 42.1 years (range 17-73), 95 had gastritis, 92 had gastric ulcers, 108 had duodenal ulcers, and 65 had gastric cancer. Using the gastritis group as a reference a significantly aberrant gene distribution was observed for the tnpA (Relative risk: 1.45; 95% CI 1.04-1.93), the cagA (Relative risk: 1.81; 95% CI 1.44-2.29), but not the tnpB gene in the gastric cancer group.CONCLUSIONS: The increased incidence of the tnpA gene in gastric cancer patients suggests a role of the tnpA gene in the development of H. pylori induced gastric cancer.

AB - BACKGROUND: Numerous proteins have been proposed as virulence factors for the gram negative gastric bacterium Helicobacter pylori but only for a few this has unequivocally been demonstrated. The aim of the current study was to evaluate the association of the putative virulence factors tnpA and tnpB (no cagA) with H. pylori associated gastroduodenal diseases.METHODS: A PCR based assay was used to determine the presence of the tnpA and tnpB genes, as well as of cagA, in 360H. pylori strains isolated from H. pylori infected patients.RESULTS: Of 360 H. pylori culture positive patients (196 men, 164 women; average age 42.1 years (range 17-73), 95 had gastritis, 92 had gastric ulcers, 108 had duodenal ulcers, and 65 had gastric cancer. Using the gastritis group as a reference a significantly aberrant gene distribution was observed for the tnpA (Relative risk: 1.45; 95% CI 1.04-1.93), the cagA (Relative risk: 1.81; 95% CI 1.44-2.29), but not the tnpB gene in the gastric cancer group.CONCLUSIONS: The increased incidence of the tnpA gene in gastric cancer patients suggests a role of the tnpA gene in the development of H. pylori induced gastric cancer.

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KW - Duodenal Ulcer

KW - Female

KW - Gastritis

KW - Helicobacter Infections

KW - Helicobacter pylori

KW - Humans

KW - Iran

KW - Male

KW - Middle Aged

KW - Stomach Neoplasms

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DO - 10.1186/1471-230X-14-33

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SN - 1471-230X

VL - 14

JO - BMC Gastroenterology

JF - BMC Gastroenterology

M1 - 14:33

ER -

Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori

Abstract

Keywords

UN SDGs

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