Cleavage inhibition of the murine coronavirus spike protein by a furin-like enzyme affects cell-cell but not virus-cell fusion

Cornelis A M de Haan; Konrad Stadler; Gert-Jan Godeke; Berend Jan Bosch; Peter J M Rottier

doi:10.1128/JVI.78.11.6048-6054.2004

Cleavage inhibition of the murine coronavirus spike protein by a furin-like enzyme affects cell-cell but not virus-cell fusion

Cornelis A M de Haan, Konrad Stadler, Gert-Jan Godeke, Berend Jan Bosch, Peter J M Rottier

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Cleavage of the mouse hepatitis coronavirus strain A59 spike protein was blocked in a concentration-dependent manner by a peptide furin inhibitor, indicating that furin or a furin-like enzyme is responsible for this process. While cell-cell fusion was clearly affected by preventing spike protein cleavage, virus-cell fusion was not, indicating that these events have different requirements.

Original language	English
Pages (from-to)	6048-54
Number of pages	7
Journal	Journal of Virology
Volume	78
Issue number	11
DOIs	https://doi.org/10.1128/JVI.78.11.6048-6054.2004
Publication status	Published - Jun 2004

Keywords

Endocytosis
Furin
Hydrogen-Ion Concentration
Membrane Fusion
Membrane Glycoproteins
Spike Glycoprotein, Coronavirus
Viral Envelope Proteins

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JVI.78.11.6048-6054.2004

Journal of Virology-2004-de Haan-6048.fullFinal published version, 1 MB

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abstract = "Cleavage of the mouse hepatitis coronavirus strain A59 spike protein was blocked in a concentration-dependent manner by a peptide furin inhibitor, indicating that furin or a furin-like enzyme is responsible for this process. While cell-cell fusion was clearly affected by preventing spike protein cleavage, virus-cell fusion was not, indicating that these events have different requirements.",

keywords = "Endocytosis, Furin, Hydrogen-Ion Concentration, Membrane Fusion, Membrane Glycoproteins, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins",

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AU - de Haan, Cornelis A M

AU - Stadler, Konrad

AU - Godeke, Gert-Jan

AU - Bosch, Berend Jan

AU - Rottier, Peter J M

PY - 2004/6

Y1 - 2004/6

N2 - Cleavage of the mouse hepatitis coronavirus strain A59 spike protein was blocked in a concentration-dependent manner by a peptide furin inhibitor, indicating that furin or a furin-like enzyme is responsible for this process. While cell-cell fusion was clearly affected by preventing spike protein cleavage, virus-cell fusion was not, indicating that these events have different requirements.

AB - Cleavage of the mouse hepatitis coronavirus strain A59 spike protein was blocked in a concentration-dependent manner by a peptide furin inhibitor, indicating that furin or a furin-like enzyme is responsible for this process. While cell-cell fusion was clearly affected by preventing spike protein cleavage, virus-cell fusion was not, indicating that these events have different requirements.

KW - Endocytosis

KW - Furin

KW - Hydrogen-Ion Concentration

KW - Membrane Fusion

KW - Membrane Glycoproteins

KW - Spike Glycoprotein, Coronavirus

KW - Viral Envelope Proteins

U2 - 10.1128/JVI.78.11.6048-6054.2004

DO - 10.1128/JVI.78.11.6048-6054.2004

M3 - Article

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JO - Journal of Virology

JF - Journal of Virology

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ER -

Cleavage inhibition of the murine coronavirus spike protein by a furin-like enzyme affects cell-cell but not virus-cell fusion

Abstract

Keywords

UN SDGs

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