Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury

Janine C Deddens; Krijn R Vrijsen; Johanna M Colijn; Martinus I Oerlemans; Corina H G Metz; Els J van der Vlist; Esther N M Nolte-'t Hoen; Krista den Ouden; Sanne J Jansen Of Lorkeers; Tycho I G van der Spoel; Stefan Koudstaal; Ger J Arkesteijn; Marca H M Wauben; Linda W van Laake; Pieter A Doevendans; Steven A J Chamuleau; Joost P G Sluijter

doi:10.1007/s12265-016-9705-1

Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury

Janine C Deddens
, Krijn R Vrijsen
, Johanna M Colijn
, Martinus I Oerlemans
, Corina H G Metz
, Els J van der Vlist
, Esther N M Nolte-'t Hoen
, Krista den Ouden
, Sanne J Jansen Of Lorkeers
, Tycho I G van der Spoel
, Stefan Koudstaal
, Ger J Arkesteijn
, Marca H M Wauben
, Linda W van Laake
, Pieter A Doevendans
, Steven A J Chamuleau
, Joost P G Sluijter

Pharmacology

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Plasma-circulating microRNAs have been implicated as novel early biomarkers for myocardial infarction (MI) due to their high specificity for cardiac injury. For swift clinical translation of this potential biomarker, it is important to understand their temporal and spatial characteristics upon MI. Therefore, we studied the temporal release, potential source, and transportation of circulating miRNAs in different models of ischemia reperfusion (I/R) injury. We demonstrated that extracellular vesicles are released from the ischemic myocardium upon I/R injury. Moreover, we provided evidence that cardiac and muscle-specific miRNAs are transported by extracellular vesicles and are rapidly detectable in plasma. Since these vesicles are enriched for the released miRNAs and their detection precedes traditional damage markers, they hold great potential as specific early biomarkers for MI.

Original language	English
Pages (from-to)	291-301
Journal	Journal of Cardiovascular Translational Research
Volume	9
Issue number	4
DOIs	https://doi.org/10.1007/s12265-016-9705-1
Publication status	Published - 6 Jul 2016

Keywords

Circulating microRNA
Biomarkers
Myocardial infarction
Extracellular vesicles
Exosomes

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Deddens, J. C., Vrijsen, K. R., Colijn, J. M., Oerlemans, M. I., Metz, C. H. G., van der Vlist, E. J., Nolte-'t Hoen, E. N. M., den Ouden, K., Jansen Of Lorkeers, S. J., van der Spoel, T. I. G., Koudstaal, S., Arkesteijn, G. J., Wauben, M. H. M., van Laake, L. W., Doevendans, P. A., Chamuleau, S. A. J., & Sluijter, J. P. G. (2016). Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury. Journal of Cardiovascular Translational Research, 9(4), 291-301. https://doi.org/10.1007/s12265-016-9705-1

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title = "Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury",

abstract = "Plasma-circulating microRNAs have been implicated as novel early biomarkers for myocardial infarction (MI) due to their high specificity for cardiac injury. For swift clinical translation of this potential biomarker, it is important to understand their temporal and spatial characteristics upon MI. Therefore, we studied the temporal release, potential source, and transportation of circulating miRNAs in different models of ischemia reperfusion (I/R) injury. We demonstrated that extracellular vesicles are released from the ischemic myocardium upon I/R injury. Moreover, we provided evidence that cardiac and muscle-specific miRNAs are transported by extracellular vesicles and are rapidly detectable in plasma. Since these vesicles are enriched for the released miRNAs and their detection precedes traditional damage markers, they hold great potential as specific early biomarkers for MI.",

keywords = "Circulating microRNA, Biomarkers, Myocardial infarction, Extracellular vesicles, Exosomes",

author = "Deddens, \{Janine C\} and Vrijsen, \{Krijn R\} and Colijn, \{Johanna M\} and Oerlemans, \{Martinus I\} and Metz, \{Corina H G\} and \{van der Vlist\}, \{Els J\} and \{Nolte-'t Hoen\}, \{Esther N M\} and \{den Ouden\}, Krista and \{Jansen Of Lorkeers\}, \{Sanne J\} and \{van der Spoel\}, \{Tycho I G\} and Stefan Koudstaal and Arkesteijn, \{Ger J\} and Wauben, \{Marca H M\} and \{van Laake\}, \{Linda W\} and Doevendans, \{Pieter A\} and Chamuleau, \{Steven A J\} and Sluijter, \{Joost P G\}",

year = "2016",

month = jul,

day = "6",

doi = "10.1007/s12265-016-9705-1",

language = "English",

volume = "9",

pages = "291--301",

journal = "Journal of Cardiovascular Translational Research",

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Deddens, JC, Vrijsen, KR, Colijn, JM, Oerlemans, MI, Metz, CHG, van der Vlist, EJ, Nolte-'t Hoen, ENM, den Ouden, K, Jansen Of Lorkeers, SJ, van der Spoel, TIG, Koudstaal, S, Arkesteijn, GJ, Wauben, MHM, van Laake, LW, Doevendans, PA, Chamuleau, SAJ & Sluijter, JPG 2016, 'Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury', Journal of Cardiovascular Translational Research, vol. 9, no. 4, pp. 291-301. https://doi.org/10.1007/s12265-016-9705-1

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T1 - Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury

AU - Deddens, Janine C

AU - Vrijsen, Krijn R

AU - Colijn, Johanna M

AU - Oerlemans, Martinus I

AU - Metz, Corina H G

AU - van der Vlist, Els J

AU - Nolte-'t Hoen, Esther N M

AU - den Ouden, Krista

AU - Jansen Of Lorkeers, Sanne J

AU - van der Spoel, Tycho I G

AU - Koudstaal, Stefan

AU - Arkesteijn, Ger J

AU - Wauben, Marca H M

AU - van Laake, Linda W

AU - Doevendans, Pieter A

AU - Chamuleau, Steven A J

AU - Sluijter, Joost P G

PY - 2016/7/6

Y1 - 2016/7/6

N2 - Plasma-circulating microRNAs have been implicated as novel early biomarkers for myocardial infarction (MI) due to their high specificity for cardiac injury. For swift clinical translation of this potential biomarker, it is important to understand their temporal and spatial characteristics upon MI. Therefore, we studied the temporal release, potential source, and transportation of circulating miRNAs in different models of ischemia reperfusion (I/R) injury. We demonstrated that extracellular vesicles are released from the ischemic myocardium upon I/R injury. Moreover, we provided evidence that cardiac and muscle-specific miRNAs are transported by extracellular vesicles and are rapidly detectable in plasma. Since these vesicles are enriched for the released miRNAs and their detection precedes traditional damage markers, they hold great potential as specific early biomarkers for MI.

AB - Plasma-circulating microRNAs have been implicated as novel early biomarkers for myocardial infarction (MI) due to their high specificity for cardiac injury. For swift clinical translation of this potential biomarker, it is important to understand their temporal and spatial characteristics upon MI. Therefore, we studied the temporal release, potential source, and transportation of circulating miRNAs in different models of ischemia reperfusion (I/R) injury. We demonstrated that extracellular vesicles are released from the ischemic myocardium upon I/R injury. Moreover, we provided evidence that cardiac and muscle-specific miRNAs are transported by extracellular vesicles and are rapidly detectable in plasma. Since these vesicles are enriched for the released miRNAs and their detection precedes traditional damage markers, they hold great potential as specific early biomarkers for MI.

KW - Circulating microRNA

KW - Biomarkers

KW - Myocardial infarction

KW - Extracellular vesicles

KW - Exosomes

U2 - 10.1007/s12265-016-9705-1

DO - 10.1007/s12265-016-9705-1

M3 - Article

C2 - 27383837

SN - 1937-5387

VL - 9

SP - 291

EP - 301

JO - Journal of Cardiovascular Translational Research

JF - Journal of Cardiovascular Translational Research

IS - 4

ER -

Circulating Extracellular Vesicles Contain miRNAs and are Released as Early Biomarkers for Cardiac Injury

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Keywords

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