Chicken mannose binding lectin has antiviral activity towards infectious bronchitis virus

Weidong Zhang; Kim M Bouwman; Steven J van Beurden; Soledad R Ordonez; Martin van Eijk; Henk P Haagsman; M Hélène Verheije; Edwin J A Veldhuizen

doi:10.1016/j.virol.2017.06.028

Chicken mannose binding lectin has antiviral activity towards infectious bronchitis virus

Weidong Zhang, Kim M Bouwman, Steven J van Beurden, Soledad R Ordonez, Martin van Eijk, Henk P Haagsman, M Hélène Verheije, Edwin J A Veldhuizen

Utrecht University

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Mannose binding lectin (MBL) is a collagenous C-type lectin, which plays an important role in innate immunity. It can bind to carbohydrates on the surface of a wide range of pathogens, including viruses. Here we studied the antiviral effect of recombinant chicken (rc)MBL against Infectious Bronchitis Virus (IBV), a highly contagious coronavirus of chicken. rcMBL inhibited in a dose-dependent manner the infection of BHK-21 cells by IBV-Beaudette, as detected by immunofluorescence staining of viral proteins and qPCR. ELISA and negative staining electron microscopy showed that rcMBL bound directly to IBV, resulting in the aggregation of viral particles. Furthermore, we demonstrated that MBL bound specifically to the spike S1 protein of IBV which mediates viral attachment. This subsequently blocked the attachment of S1 to IBV-susceptible cells in chicken tracheal tissues as shown in protein histochemistry. Taken together, rcMBL exhibits antiviral activity against IBV, based on a direct interaction with IBV virions.

Original language	English
Pages (from-to)	252-259
Number of pages	8
Journal	Virology
Volume	509
DOIs	https://doi.org/10.1016/j.virol.2017.06.028
Publication status	Published - Sept 2017

Keywords

Animals
Antiviral Agents
Cell Line
Chickens
Cricetinae
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
Immunity, Innate
Infectious bronchitis virus
Mannose-Binding Lectin
Microscopy, Electron, Transmission
Protein Binding
Real-Time Polymerase Chain Reaction
Spike Glycoprotein, Coronavirus
Virion
Virus Attachment
Journal Article

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10.1016/j.virol.2017.06.028

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title = "Chicken mannose binding lectin has antiviral activity towards infectious bronchitis virus",

abstract = "Mannose binding lectin (MBL) is a collagenous C-type lectin, which plays an important role in innate immunity. It can bind to carbohydrates on the surface of a wide range of pathogens, including viruses. Here we studied the antiviral effect of recombinant chicken (rc)MBL against Infectious Bronchitis Virus (IBV), a highly contagious coronavirus of chicken. rcMBL inhibited in a dose-dependent manner the infection of BHK-21 cells by IBV-Beaudette, as detected by immunofluorescence staining of viral proteins and qPCR. ELISA and negative staining electron microscopy showed that rcMBL bound directly to IBV, resulting in the aggregation of viral particles. Furthermore, we demonstrated that MBL bound specifically to the spike S1 protein of IBV which mediates viral attachment. This subsequently blocked the attachment of S1 to IBV-susceptible cells in chicken tracheal tissues as shown in protein histochemistry. Taken together, rcMBL exhibits antiviral activity against IBV, based on a direct interaction with IBV virions.",

keywords = "Animals, Antiviral Agents, Cell Line, Chickens, Cricetinae, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Immunity, Innate, Infectious bronchitis virus, Mannose-Binding Lectin, Microscopy, Electron, Transmission, Protein Binding, Real-Time Polymerase Chain Reaction, Spike Glycoprotein, Coronavirus, Virion, Virus Attachment, Journal Article",

author = "Weidong Zhang and Bouwman, {Kim M} and {van Beurden}, {Steven J} and Ordonez, {Soledad R} and {van Eijk}, Martin and Haagsman, {Henk P} and Verheije, {M H{\'e}l{\`e}ne} and Veldhuizen, {Edwin J A}",

year = "2017",

month = sep,

doi = "10.1016/j.virol.2017.06.028",

language = "English",

volume = "509",

pages = "252--259",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

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T1 - Chicken mannose binding lectin has antiviral activity towards infectious bronchitis virus

AU - Zhang, Weidong

AU - Bouwman, Kim M

AU - van Beurden, Steven J

AU - Ordonez, Soledad R

AU - van Eijk, Martin

AU - Haagsman, Henk P

AU - Verheije, M Hélène

AU - Veldhuizen, Edwin J A

PY - 2017/9

Y1 - 2017/9

N2 - Mannose binding lectin (MBL) is a collagenous C-type lectin, which plays an important role in innate immunity. It can bind to carbohydrates on the surface of a wide range of pathogens, including viruses. Here we studied the antiviral effect of recombinant chicken (rc)MBL against Infectious Bronchitis Virus (IBV), a highly contagious coronavirus of chicken. rcMBL inhibited in a dose-dependent manner the infection of BHK-21 cells by IBV-Beaudette, as detected by immunofluorescence staining of viral proteins and qPCR. ELISA and negative staining electron microscopy showed that rcMBL bound directly to IBV, resulting in the aggregation of viral particles. Furthermore, we demonstrated that MBL bound specifically to the spike S1 protein of IBV which mediates viral attachment. This subsequently blocked the attachment of S1 to IBV-susceptible cells in chicken tracheal tissues as shown in protein histochemistry. Taken together, rcMBL exhibits antiviral activity against IBV, based on a direct interaction with IBV virions.

AB - Mannose binding lectin (MBL) is a collagenous C-type lectin, which plays an important role in innate immunity. It can bind to carbohydrates on the surface of a wide range of pathogens, including viruses. Here we studied the antiviral effect of recombinant chicken (rc)MBL against Infectious Bronchitis Virus (IBV), a highly contagious coronavirus of chicken. rcMBL inhibited in a dose-dependent manner the infection of BHK-21 cells by IBV-Beaudette, as detected by immunofluorescence staining of viral proteins and qPCR. ELISA and negative staining electron microscopy showed that rcMBL bound directly to IBV, resulting in the aggregation of viral particles. Furthermore, we demonstrated that MBL bound specifically to the spike S1 protein of IBV which mediates viral attachment. This subsequently blocked the attachment of S1 to IBV-susceptible cells in chicken tracheal tissues as shown in protein histochemistry. Taken together, rcMBL exhibits antiviral activity against IBV, based on a direct interaction with IBV virions.

KW - Animals

KW - Antiviral Agents

KW - Cell Line

KW - Chickens

KW - Cricetinae

KW - Enzyme-Linked Immunosorbent Assay

KW - Fluorescent Antibody Technique

KW - Immunity, Innate

KW - Infectious bronchitis virus

KW - Mannose-Binding Lectin

KW - Microscopy, Electron, Transmission

KW - Protein Binding

KW - Real-Time Polymerase Chain Reaction

KW - Spike Glycoprotein, Coronavirus

KW - Virion

KW - Virus Attachment

KW - Journal Article

U2 - 10.1016/j.virol.2017.06.028

DO - 10.1016/j.virol.2017.06.028

M3 - Article

C2 - 28686880

SN - 0042-6822

VL - 509

SP - 252

EP - 259

JO - Virology

JF - Virology

ER -

Chicken mannose binding lectin has antiviral activity towards infectious bronchitis virus

Abstract

Keywords

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