Characterization of the tobacco eIF-4A gene family

G W Owttrim; T Mandel; H Trachsel; A A Thomas; C Kuhlemeier

Characterization of the tobacco eIF-4A gene family

G W Owttrim
, T Mandel
, H Trachsel
, A A Thomas
, C Kuhlemeier

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Characterization of cDNAs encoding eukaryotic translation initiation factor 4A (eIF-4A) indicates the expression of a minimum of ten related genes in tobacco leaf cells. The ten groups fall into two gene families, NeIF-4A2 and NeIF-4A3. The majority of the cDNAs exhibit significant sequence similarity to the NeIF-4A2 family at both the DNA and deduced amino acid levels. Northern analysis using specific probes indicates variable expression of four family members in various tobacco organs. Western analysis, using an anti-tobacco eIF-4A polyclonal antibody, reveals a complex pattern of immunologically related polypeptides of approximately 46 kDa. Subcellular fractionation suggests that at least one eIF-4A-related polypeptide is located in the chloroplast where it is ribosome-associated.

Original language	English
Pages (from-to)	1747-57
Number of pages	11
Journal	Plant Molecular Biology
Volume	26
Issue number	6
Publication status	Published - Dec 1994

Keywords

Amino Acid Sequence
Base Sequence
Blotting, Western
Cell Compartmentation
DNA, Complementary
Eukaryotic Initiation Factor-4A
Gene Dosage
Gene Library
Genes, Plant
Molecular Sequence Data
Multigene Family
Nucleic Acid Hybridization
Peptide Initiation Factors
Plants, Toxic
Seeds
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Tissue Distribution
Tobacco
Triticum

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@article{0619a66ca46c43a4b979c5b3b4167745,

title = "Characterization of the tobacco eIF-4A gene family",

abstract = "Characterization of cDNAs encoding eukaryotic translation initiation factor 4A (eIF-4A) indicates the expression of a minimum of ten related genes in tobacco leaf cells. The ten groups fall into two gene families, NeIF-4A2 and NeIF-4A3. The majority of the cDNAs exhibit significant sequence similarity to the NeIF-4A2 family at both the DNA and deduced amino acid levels. Northern analysis using specific probes indicates variable expression of four family members in various tobacco organs. Western analysis, using an anti-tobacco eIF-4A polyclonal antibody, reveals a complex pattern of immunologically related polypeptides of approximately 46 kDa. Subcellular fractionation suggests that at least one eIF-4A-related polypeptide is located in the chloroplast where it is ribosome-associated.",

keywords = "Amino Acid Sequence, Base Sequence, Blotting, Western, Cell Compartmentation, DNA, Complementary, Eukaryotic Initiation Factor-4A, Gene Dosage, Gene Library, Genes, Plant, Molecular Sequence Data, Multigene Family, Nucleic Acid Hybridization, Peptide Initiation Factors, Plants, Toxic, Seeds, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Tissue Distribution, Tobacco, Triticum",

author = "Owttrim, \{G W\} and T Mandel and H Trachsel and Thomas, \{A A\} and C Kuhlemeier",

year = "1994",

month = dec,

language = "English",

volume = "26",

pages = "1747--57",

journal = "Plant Molecular Biology",

issn = "0167-4412",

publisher = "Springer Science and Business Media B.V.",

number = "6",

}

TY - JOUR

T1 - Characterization of the tobacco eIF-4A gene family

AU - Owttrim, G W

AU - Mandel, T

AU - Trachsel, H

AU - Thomas, A A

AU - Kuhlemeier, C

PY - 1994/12

Y1 - 1994/12

N2 - Characterization of cDNAs encoding eukaryotic translation initiation factor 4A (eIF-4A) indicates the expression of a minimum of ten related genes in tobacco leaf cells. The ten groups fall into two gene families, NeIF-4A2 and NeIF-4A3. The majority of the cDNAs exhibit significant sequence similarity to the NeIF-4A2 family at both the DNA and deduced amino acid levels. Northern analysis using specific probes indicates variable expression of four family members in various tobacco organs. Western analysis, using an anti-tobacco eIF-4A polyclonal antibody, reveals a complex pattern of immunologically related polypeptides of approximately 46 kDa. Subcellular fractionation suggests that at least one eIF-4A-related polypeptide is located in the chloroplast where it is ribosome-associated.

AB - Characterization of cDNAs encoding eukaryotic translation initiation factor 4A (eIF-4A) indicates the expression of a minimum of ten related genes in tobacco leaf cells. The ten groups fall into two gene families, NeIF-4A2 and NeIF-4A3. The majority of the cDNAs exhibit significant sequence similarity to the NeIF-4A2 family at both the DNA and deduced amino acid levels. Northern analysis using specific probes indicates variable expression of four family members in various tobacco organs. Western analysis, using an anti-tobacco eIF-4A polyclonal antibody, reveals a complex pattern of immunologically related polypeptides of approximately 46 kDa. Subcellular fractionation suggests that at least one eIF-4A-related polypeptide is located in the chloroplast where it is ribosome-associated.

KW - Amino Acid Sequence

KW - Base Sequence

KW - Blotting, Western

KW - Cell Compartmentation

KW - DNA, Complementary

KW - Eukaryotic Initiation Factor-4A

KW - Gene Dosage

KW - Gene Library

KW - Genes, Plant

KW - Molecular Sequence Data

KW - Multigene Family

KW - Nucleic Acid Hybridization

KW - Peptide Initiation Factors

KW - Plants, Toxic

KW - Seeds

KW - Sequence Analysis, DNA

KW - Sequence Homology, Amino Acid

KW - Sequence Homology, Nucleic Acid

KW - Tissue Distribution

KW - Tobacco

KW - Triticum

M3 - Article

C2 - 7858215

SN - 0167-4412

VL - 26

SP - 1747

EP - 1757

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 6

ER -

Characterization of the tobacco eIF-4A gene family

Abstract

Keywords

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