TY - JOUR
T1 - Characterization of the ferret TRB locus guided by V, D, J, and C gene expression analysis
AU - Gerritsen, Bram
AU - Pandit, Aridaman
AU - Zaaraoui-Boutahar, Fatiha
AU - van den Hout, Mirjam C G N
AU - van IJcken, Wilfred F J
AU - de Boer, Rob J
AU - Andeweg, Arno C
PY - 2019/12/3
Y1 - 2019/12/3
N2 - The domestic ferret, Mustela putorius furo, is an important mammalian animal model to study human respiratory infection. However, insufficient genomic annotation hampers detailed studies of ferret T cell responses. In this study, we analyzed the published T cell receptor beta (TRB) locus and performed high-throughput sequencing (HTS) of peripheral blood of four healthy adult ferrets to identify expressed V, D, J, and C genes. The HTS data is used as a guide to manually curate the expressed V, D, J, and C genes. The ferret locus appears to be most similar to that of the dog. Like other mammalian TRB loci, the ferret TRB locus contains a library of variable genes located upstream of two D-J-C gene clusters, followed by a (in the ferret non-functional) V gene with an inverted transcriptional orientation. All TRB genes (expressed or not) reported here have been approved by the IMGT/WHO-IUIS nomenclature committee.
AB - The domestic ferret, Mustela putorius furo, is an important mammalian animal model to study human respiratory infection. However, insufficient genomic annotation hampers detailed studies of ferret T cell responses. In this study, we analyzed the published T cell receptor beta (TRB) locus and performed high-throughput sequencing (HTS) of peripheral blood of four healthy adult ferrets to identify expressed V, D, J, and C genes. The HTS data is used as a guide to manually curate the expressed V, D, J, and C genes. The ferret locus appears to be most similar to that of the dog. Like other mammalian TRB loci, the ferret TRB locus contains a library of variable genes located upstream of two D-J-C gene clusters, followed by a (in the ferret non-functional) V gene with an inverted transcriptional orientation. All TRB genes (expressed or not) reported here have been approved by the IMGT/WHO-IUIS nomenclature committee.
KW - T cell receptor
KW - TRB locus
KW - Ferret (Mustela putorius furo)
KW - IMGT
KW - Comparative genomics
KW - Immune repertoire sequencing
U2 - 10.1007/s00251-019-01142-9
DO - 10.1007/s00251-019-01142-9
M3 - Review article
C2 - 31797007
SN - 0093-7711
JO - Immunogenetics
JF - Immunogenetics
ER -