TY - JOUR
T1 - Characterization and comparison of canine multipotent stromal cells derived from liver and bone marrow
AU - Malagola, Ermanno
AU - Teunissen, Michelle
AU - van der Laan, Luc J W
AU - Verstegen, Monique
AU - Schotanus, Baukje Akke
AU - van Steenbeek, Frank G
AU - Penning, Louis C
AU - van Wolferen, Monique E
AU - Tryfonidou, Marianna A
AU - Spee, Bart
PY - 2016
Y1 - 2016
N2 - Liver-derived multipotent stromal cells (L-MSCs) may prove preferable for treatment strategies of liver diseases, in comparison to the widely studied bone marrow-derived MSCs (BM-MSCs). Canines are a large animal model, in which the pathologies of liver diseases is similar to man. This study further promotes the implementation of canine models in MSC-based treatments of liver diseases. L-MSCs were characterized and compared to BM-MSCs from the same individual. Both cell types demonstrated a spindle-shape fibroblast-like morphology, possessed the same growth potential, and demonstrated similar immunomodulation gene expression of CD274, PTGS-1 and -2. Marked differences in cell surface markers, CD105 and CD146, distinguished these two cell populations, and L-MSCs retained a liver-specific imprinting, observed by expression of CK18 and CK19. Finally, both populations differentiated towards the osteogenic and adipogenic lineage, however L-MSCs failed to differentiate into the chondrogenic lineage. In conclusion, characterization of canine L-MSCs and BM-MSCs demonstrated that the two cell type populations are highly comparable. Although it is still unclear which cell source is preferred for clinical application in liver treatment strategies, this study provides a foundation for future controlled studies with MSC cell therapy in various liver diseases in dogs before their application in man.
AB - Liver-derived multipotent stromal cells (L-MSCs) may prove preferable for treatment strategies of liver diseases, in comparison to the widely studied bone marrow-derived MSCs (BM-MSCs). Canines are a large animal model, in which the pathologies of liver diseases is similar to man. This study further promotes the implementation of canine models in MSC-based treatments of liver diseases. L-MSCs were characterized and compared to BM-MSCs from the same individual. Both cell types demonstrated a spindle-shape fibroblast-like morphology, possessed the same growth potential, and demonstrated similar immunomodulation gene expression of CD274, PTGS-1 and -2. Marked differences in cell surface markers, CD105 and CD146, distinguished these two cell populations, and L-MSCs retained a liver-specific imprinting, observed by expression of CK18 and CK19. Finally, both populations differentiated towards the osteogenic and adipogenic lineage, however L-MSCs failed to differentiate into the chondrogenic lineage. In conclusion, characterization of canine L-MSCs and BM-MSCs demonstrated that the two cell type populations are highly comparable. Although it is still unclear which cell source is preferred for clinical application in liver treatment strategies, this study provides a foundation for future controlled studies with MSC cell therapy in various liver diseases in dogs before their application in man.
U2 - 10.1089/scd.2015.0125
DO - 10.1089/scd.2015.0125
M3 - Article
C2 - 26462417
SN - 1547-3287
VL - 35
JO - Stem Cells and Development
JF - Stem Cells and Development
IS - 2
ER -