cGAS and STING knockout rescues virus infection of plasmid DNA-transfected cells

Martijn A Langereis; Huib H Rabouw; Melle Holwerda; Linda J Visser; Frank J M van Kuppeveld

doi:10.1128/JVI.01781-15

cGAS and STING knockout rescues virus infection of plasmid DNA-transfected cells

Martijn A Langereis, Huib H Rabouw, Melle Holwerda, Linda J Visser, Frank J M van Kuppeveld

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid CRISPR/Cas9 system, we generated cGAS/STING knockout cells and show that these cells can as efficiently be infected after plasmid DNA transfection as non-transfected cells.

Original language	English
Pages (from-to)	11169-11173
Journal	Journal of Virology
Volume	89
Issue number	21
DOIs	https://doi.org/10.1128/JVI.01781-15
Publication status	Published - 26 Aug 2015

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abstract = "It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid CRISPR/Cas9 system, we generated cGAS/STING knockout cells and show that these cells can as efficiently be infected after plasmid DNA transfection as non-transfected cells.",

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AU - Holwerda, Melle

AU - Visser, Linda J

AU - van Kuppeveld, Frank J M

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AB - It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid CRISPR/Cas9 system, we generated cGAS/STING knockout cells and show that these cells can as efficiently be infected after plasmid DNA transfection as non-transfected cells.

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JO - Journal of Virology

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cGAS and STING knockout rescues virus infection of plasmid DNA-transfected cells

Abstract

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