Cellular solid-state NMR spectroscopy

Reinier Damman, Siddarth Narasimhan, Markus Weingarth, Marc Baldus*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review


Increasing evidence suggests that the highly complex and dynamic environment of bacterial and human cells imposes critical control on cellular functions which are difficult to mimic under in vitro conditions. Complementary to high-resolution light microscopy and electron tomography, in-cell solution-state NMR can track such structural and dynamical interactions at the most detailed, i.e., atomic level, provided that the molecular units tumble rapidly. Cellular solid-state NMR (ssNMR), on the other hand, provides increasing possibilities to probe molecular structure in bacterial and human cell preparations largely irrespective of molecular size. Here we review preparative as well as methodological aspects of such studies and highlight recent applications focusing on proteins embedded in their natural cell membrane environment.
Original languageEnglish
Title of host publicationIn-cell NMR Spectroscopy
Subtitle of host publicationFrom Molecular Sciences to Cell Biology
EditorsYutaka Ito, Volker Dotsch, Masahiro Shirakawa
PublisherRoyal Society of Chemistry
Number of pages21
ISBN (Electronic)978-1-78801-307-9
ISBN (Print)978-1-78801-217-1
Publication statusPublished - 1 Jan 2020

Publication series

NameNew Developments in NMR
ISSN (Print)2044-253X
ISSN (Electronic)2044-2548


Dive into the research topics of 'Cellular solid-state NMR spectroscopy'. Together they form a unique fingerprint.

Cite this