Cellular Cryo-Electron Tomography

Jo H. Judernatz; Marc C. Roelofs; Tzviya Zeev-Ben-Mordehai

doi:10.1016/B978-0-12-821618-7.00112-7

Cellular Cryo-Electron Tomography

Jo H. Judernatz, Marc C. Roelofs, Tzviya Zeev-Ben-Mordehai

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

Electron tomography at cryogenic temperatures is becoming a prominent tool in cell biology. It provides three-dimensional detailed information on cellular organization. Advanced image processing of tomogram sub-volumes allows near-atomic in-cell macromolecules structure determination. Here we describe how cells and tissues are prepared for imaging and how data is collected and processed. Selected examples are given to demonstrate the breadth of samples that can be processed thus demonstrating the contribution of cryogenic electron tomography to cell biology; prospects for future developments are discussed.

Original language	English
Title of host publication	Encyclopedia of Cell Biology
Subtitle of host publication	Volume 1-6, Second Edition
Editors	R.A. Bradshaw, G.W. Hart, P.D. Stahl
Publisher	Elsevier
Pages	4-15
Number of pages	12
Volume	2
ISBN (Electronic)	9780128216248
DOIs	https://doi.org/10.1016/B978-0-12-821618-7.00112-7
Publication status	Published - 1 Jan 2022

Bibliographical note

Publisher Copyright:
© 2023 Elsevier Inc. All rights reserved.

Keywords

3D Reconstruction
3D Segmentation
Correlative light and electron microscopy
Cryo-electron microscopy
Cryo-electron tomography
Focused ion beam milling
In situ structural biology
Structural cell biology
Sub-tomogram averaging

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10.1016/B978-0-12-821618-7.00112-7

Cite this

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title = "Cellular Cryo-Electron Tomography",

abstract = "Electron tomography at cryogenic temperatures is becoming a prominent tool in cell biology. It provides three-dimensional detailed information on cellular organization. Advanced image processing of tomogram sub-volumes allows near-atomic in-cell macromolecules structure determination. Here we describe how cells and tissues are prepared for imaging and how data is collected and processed. Selected examples are given to demonstrate the breadth of samples that can be processed thus demonstrating the contribution of cryogenic electron tomography to cell biology; prospects for future developments are discussed.",

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AU - Judernatz, Jo H.

AU - Roelofs, Marc C.

AU - Zeev-Ben-Mordehai, Tzviya

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Y1 - 2022/1/1

N2 - Electron tomography at cryogenic temperatures is becoming a prominent tool in cell biology. It provides three-dimensional detailed information on cellular organization. Advanced image processing of tomogram sub-volumes allows near-atomic in-cell macromolecules structure determination. Here we describe how cells and tissues are prepared for imaging and how data is collected and processed. Selected examples are given to demonstrate the breadth of samples that can be processed thus demonstrating the contribution of cryogenic electron tomography to cell biology; prospects for future developments are discussed.

AB - Electron tomography at cryogenic temperatures is becoming a prominent tool in cell biology. It provides three-dimensional detailed information on cellular organization. Advanced image processing of tomogram sub-volumes allows near-atomic in-cell macromolecules structure determination. Here we describe how cells and tissues are prepared for imaging and how data is collected and processed. Selected examples are given to demonstrate the breadth of samples that can be processed thus demonstrating the contribution of cryogenic electron tomography to cell biology; prospects for future developments are discussed.

KW - 3D Reconstruction

KW - 3D Segmentation

KW - Correlative light and electron microscopy

KW - Cryo-electron microscopy

KW - Cryo-electron tomography

KW - Focused ion beam milling

KW - In situ structural biology

KW - Structural cell biology

KW - Sub-tomogram averaging

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DO - 10.1016/B978-0-12-821618-7.00112-7

M3 - Chapter

AN - SCOPUS:85158947437

VL - 2

SP - 4

EP - 15

BT - Encyclopedia of Cell Biology

A2 - Bradshaw, R.A.

A2 - Hart, G.W.

A2 - Stahl, P.D.

PB - Elsevier

ER -

Cellular Cryo-Electron Tomography

Abstract

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Keywords

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