Cellular Cryo-Electron Tomography

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Electron tomography at cryogenic temperatures is becoming a prominent tool in cell biology. It provides three-dimensional detailed information on cellular organization. Advanced image processing of tomogram sub-volumes allows near-atomic in-cell macromolecules structure determination. Here we describe how cells and tissues are prepared for imaging and how data is collected and processed. Selected examples are given to demonstrate the breadth of samples that can be processed thus demonstrating the contribution of cryogenic electron tomography to cell biology; prospects for future developments are discussed.

Original languageEnglish
Title of host publicationEncyclopedia of Cell Biology
Subtitle of host publicationVolume 1-6, Second Edition
EditorsR.A. Bradshaw, G.W. Hart, P.D. Stahl
PublisherElsevier
Pages4-15
Number of pages12
Volume2
ISBN (Electronic)9780128216248
DOIs
Publication statusPublished - 1 Jan 2022

Keywords

  • 3D Reconstruction
  • 3D Segmentation
  • Correlative light and electron microscopy
  • Cryo-electron microscopy
  • Cryo-electron tomography
  • Focused ion beam milling
  • In situ structural biology
  • Structural cell biology
  • Sub-tomogram averaging

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