Cell-cell adhesion mediated by a receptor-like protein tyrosine phosphatase

M F Gebbink, G C Zondag, R W Wubbolts, R L Beijersbergen, I van Etten, W H Moolenaar

    Research output: Contribution to journalArticleAcademicpeer-review


    Receptor-like protein tyrosine phosphatases (receptor-PTPs) represent a novel family of transmembrane proteins that are thought to play important roles in cellular regulation. They consist of a cytoplasmic catalytic region, a single transmembrane segment and an extracellular, putative ligand-binding domain, but the nature of their physiological ligands is unknown. We have recently cloned a new receptor-PTP (RPTP mu), the ectodomain of which includes an Ig-like and four fibronectin type III-like domains, suggesting that RPTP mu may be involved in cell-cell or cell-matrix interactions. To test this hypothesis, we expressed RPTP mu in insect Sf9 cells using recombinant baculovirus. We demonstrate that RPTP mu dramatically promotes cell-to-cell adhesion in a homophilic, Ca(2+)-independent manner. No adhesion is observed in Sf9 cells expressing a chimeric RPTP mu molecule containing the extracellular domain of the epidermal growth factor receptor. Furthermore, cells expressing an enzymatically inactive, point-mutated RPTP mu or a truncated form of RPTP mu, lacking the entire catalytic region, show adhesive properties indistinguishable from those of wild-type RPTP mu, indicating that the catalytic domain is not essential for RPTP mu-mediated adhesion. These results assign a physiological role for RPTP mu in signaling cell-cell recognition.

    Original languageEnglish
    Pages (from-to)16101-4
    Number of pages4
    JournalJournal of Biological Chemistry
    Issue number22
    Publication statusPublished - 5 Aug 1993


    • Animals
    • Base Sequence
    • Calcium
    • Catalysis
    • Cell Adhesion
    • Cell Line
    • DNA
    • Humans
    • Hydrogen-Ion Concentration
    • Membrane Proteins
    • Molecular Sequence Data
    • Moths
    • Protein Tyrosine Phosphatases
    • Receptor-Like Protein Tyrosine Phosphatases, Class 2
    • Receptor-Like Protein Tyrosine Phosphatases, Class 8
    • Receptors, Cell Surface


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