CD24 tracks divergent pluripotent states in mouse and human cells

Nika Shakiba; Carl A White; Yonatan Y Lipsitz; Ayako Yachie-Kinoshita; Peter D Tonge; Samer M I Hussein; Mira C Puri; Judith Elbaz; James Morrissey-Scoot; Mira Li; Javier Munoz Peralta; Marco Benevento; Ian M Rogers; Jacob H Hanna; Albert J R Heck; Bernd Wollscheid; Andras Nagy; Peter W Zandstra

doi:10.1038/ncomms8329

CD24 tracks divergent pluripotent states in mouse and human cells

Nika Shakiba, Carl A White, Yonatan Y Lipsitz, Ayako Yachie-Kinoshita, Peter D Tonge, Samer M I Hussein, Mira C Puri, Judith Elbaz, James Morrissey-Scoot, Mira Li, Javier Munoz Peralta, Marco Benevento, Ian M Rogers, Jacob H Hanna, Albert J R Heck, Bernd Wollscheid, Andras Nagy, Peter W Zandstra

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

Original language	English
Article number	7329
Number of pages	11
Journal	Nature Communications [E]
Volume	6
DOIs	https://doi.org/10.1038/ncomms8329
Publication status	Published - 16 Jun 2015

Keywords

Biological sciences
Developmental biology
Cell biology

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Shakiba, N., White, C. A., Lipsitz, Y. Y., Yachie-Kinoshita, A., Tonge, P. D., Hussein, S. M. I., Puri, M. C., Elbaz, J., Morrissey-Scoot, J., Li, M., Munoz Peralta, J., Benevento, M., Rogers, I. M., Hanna, J. H., Heck, A. J. R., Wollscheid, B., Nagy, A., & Zandstra, P. W. (2015). CD24 tracks divergent pluripotent states in mouse and human cells. Nature Communications [E], 6, Article 7329. https://doi.org/10.1038/ncomms8329

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title = "CD24 tracks divergent pluripotent states in mouse and human cells",

abstract = "Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.",

keywords = "Biological sciences, Developmental biology, Cell biology",

author = "Nika Shakiba and White, {Carl A} and Lipsitz, {Yonatan Y} and Ayako Yachie-Kinoshita and Tonge, {Peter D} and Hussein, {Samer M I} and Puri, {Mira C} and Judith Elbaz and James Morrissey-Scoot and Mira Li and {Munoz Peralta}, Javier and Marco Benevento and Rogers, {Ian M} and Hanna, {Jacob H} and Heck, {Albert J R} and Bernd Wollscheid and Andras Nagy and Zandstra, {Peter W}",

year = "2015",

month = jun,

day = "16",

doi = "10.1038/ncomms8329",

language = "English",

volume = "6",

journal = "Nature Communications [E]",

issn = "2041-1723",

publisher = "Nature Research",

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Shakiba, N, White, CA, Lipsitz, YY, Yachie-Kinoshita, A, Tonge, PD, Hussein, SMI, Puri, MC, Elbaz, J, Morrissey-Scoot, J, Li, M, Munoz Peralta, J, Benevento, M, Rogers, IM, Hanna, JH, Heck, AJR, Wollscheid, B, Nagy, A & Zandstra, PW 2015, 'CD24 tracks divergent pluripotent states in mouse and human cells', Nature Communications [E], vol. 6, 7329. https://doi.org/10.1038/ncomms8329

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T1 - CD24 tracks divergent pluripotent states in mouse and human cells

AU - Shakiba, Nika

AU - White, Carl A

AU - Lipsitz, Yonatan Y

AU - Yachie-Kinoshita, Ayako

AU - Tonge, Peter D

AU - Hussein, Samer M I

AU - Puri, Mira C

AU - Elbaz, Judith

AU - Morrissey-Scoot, James

AU - Li, Mira

AU - Munoz Peralta, Javier

AU - Benevento, Marco

AU - Rogers, Ian M

AU - Hanna, Jacob H

AU - Heck, Albert J R

AU - Wollscheid, Bernd

AU - Nagy, Andras

AU - Zandstra, Peter W

PY - 2015/6/16

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N2 - Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

AB - Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

KW - Biological sciences

KW - Developmental biology

KW - Cell biology

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DO - 10.1038/ncomms8329

M3 - Article

C2 - 26076835

SN - 2041-1723

VL - 6

JO - Nature Communications [E]

JF - Nature Communications [E]

M1 - 7329

ER -

CD24 tracks divergent pluripotent states in mouse and human cells

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Keywords

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