Cathelicidins PMAP-36, LL-37 and CATH-2 are similar peptides with different modes of action

Maaike R Scheenstra, Matthias van den Belt, Johanna L M Tjeerdsma-van Bokhoven, Viktoria A F Schneider, Soledad R Ordonez, Albert van Dijk, Edwin J A Veldhuizen, Henk P Haagsman

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Host defense peptides (HDPs) play a pivotal role in innate immunity and have, in addition to antimicrobial activity, also important immunomodulatory functions. Bacteria are less likely to develop resistance against HDPs because these peptides target and kill bacteria in multiple ways, as well as modulate the immune system. Therefore, HDPs, and derivatives thereof, are promising alternatives to traditional antibiotics. Hardly anything is known about the immunomodulatory functions of porcine cathelicidin PMAP-36. In this study, we aimed to determine both antibacterial and immunomodulatory activities of PMAP-36 comparing the properties of PMAP-36 analogs with two well-studied peptides, human LL-37 and chicken CATH-2. Transmission electron microscopy revealed different killing mechanisms of E. coli for PMAP-36, CATH-2 and LL-37. LL-37 binds LPS very weakly in contrast to PMAP-36, but it inhibits LPS activation of macrophages the strongest. The first 11 amino acids of the N-terminal side of PMAP-36 are dispensable for E. coli killing, LPS-neutralization and binding. Deletion of four additional amino acids resulted in a strong decrease in activity. The activity of full length PMAP-36 was not affected by monomerization, whereas the shorter analogs require dimerization for proper immunomodulatory activity but not for their antibacterial activity.

    Original languageEnglish
    Pages (from-to)4780
    JournalScientific Reports
    Volume9
    Issue number1
    DOIs
    Publication statusPublished - 2019

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