Capillary electrophoresis-mass spectrometry using an in-line sol-gel concentrator for the determination of methionine enkephalin in cerebrospinal fluid

R. Ramautar, C.K. Ratnayake, G.W. Somsen, G.J. de Jong

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In this study, a CE-MS method using a monolithic sol-gel concentrator for in-line solid-phase extraction (SPE) is evaluated for the analysis of methionine enkephalin in biological samples. Operational SPE parameters such as sample pH, loading volume, elution volume and composition have been studied. After optimization of the in-line preconcentration methodology, a 40-fold preconcentration was demonstrated for a methionine enkephalin test solution using a loading volume of 3200 nL. The method was linear in the range from 62.5 to 1000 ng/mL (R-2 > 0.99). R.S.D. values for migration times and peak areas were 1.2% and 8.4%, respectively. Finally, the analysis of cerebrospinal fluid samples spiked with methionine enkephalin and deproteinized with perchloric acid (1: 1, v/v) showed a detection limit (S/N = 3) of approximately 1 ng/mL (ca. 5 nM). The recoveries of methionine enkephalin for three concentration levels (100, 10 and I ng/mL) were in the range of 74-91%, demonstrating the promising potential of the methodology for the analysis of biological samples. (C) 2008 Elsevier B.V. All rights reserved.
Original languageUndefined/Unknown
Pages (from-to)638-642
Number of pages5
JournalTalanta
Volume78
Issue number2
Publication statusPublished - 2009

Keywords

  • Farmacie/Biofarmaceutische wetenschappen (FARM)
  • Medical technology
  • Farmacie(FARM)
  • Bescherming en bevordering van de menselijke gezondheid
  • Biomedische technologie en medicijnen
  • Public Health
  • Analytical chemistry
  • Drugmisbruik en verslaving

Cite this