Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles

Collette Fitzgerald; Zheng Chao Tu; Mary Patrick; Tracy Stiles; Andy J Lawson; Monica Santovenia; Maarten J Gilbert; Marcel van Bergen; Kevin Joyce; Janet Pruckler; Steven Stroika; Birgitta Duim; William G Miller; Vladimir Loparev; Jan C Sinnige; Patricia I Fields; Robert V Tauxe; Martin J Blaser; Jaap A Wagenaar

doi:10.1099/ijs.0.057778-0

Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles

Collette Fitzgerald
, Zheng Chao Tu
, Mary Patrick
, Tracy Stiles
, Andy J Lawson
, Monica Santovenia
, Maarten J Gilbert
, Marcel van Bergen
, Kevin Joyce
, Janet Pruckler
, Steven Stroika
, Birgitta Duim
, William G Miller
, Vladimir Loparev
, Jan C Sinnige
, Patricia I Fields
, Robert V Tauxe
, Martin J Blaser
, Jaap A Wagenaar

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetus-like strains from humans (n = 8) and reptiles (n = 5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and genomic data from sap analysis, 16S rRNA gene and hsp60 sequence comparison, pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, DNA-DNA hybridization and whole genome sequencing demonstrated that these strains are closely related to C. fetus but clearly differentiated from recognized subspecies of C. fetus. Therefore, this unique cluster of 13 strains represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427(T) ( = ATCC BAA-2539(T) = LMG 27499(T)) as the type strain. Although this novel taxon could not be differentiated from C. fetus subsp. fetus and C. fetus subsp. venerealis using conventional phenotypic tests, MALDI-TOF MS revealed the presence of multiple phenotypic biomarkers which distinguish Campylobacter fetus subsp. testudinum subsp. nov. from recognized subspecies of C. fetus.

Original language	English
Pages (from-to)	2944-2948
Number of pages	5
Journal	International journal of systemic and evolutionary microbiology
Volume	64
Issue number	Pt 9
DOIs	https://doi.org/10.1099/ijs.0.057778-0
Publication status	Published - Sept 2014

Keywords

Amplified Fragment Length Polymorphism Analysis
Animals
Bacterial Typing Techniques
Campylobacter fetus
DNA, Bacterial
Humans
Molecular Sequence Data
Nucleic Acid Hybridization
Phylogeny
RNA, Ribosomal, 16S
Reptiles
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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Fitzgerald, C., Tu, Z. C., Patrick, M., Stiles, T., Lawson, A. J., Santovenia, M., Gilbert, M. J., van Bergen, M., Joyce, K., Pruckler, J., Stroika, S., Duim, B., Miller, W. G., Loparev, V., Sinnige, J. C., Fields, P. I., Tauxe, R. V., Blaser, M. J., & Wagenaar, J. A. (2014). Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles. International journal of systemic and evolutionary microbiology, 64(Pt 9), 2944-2948. https://doi.org/10.1099/ijs.0.057778-0

@article{f2ebfadb64514184a045dd39088e549f,

title = "Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles",

abstract = "A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetus-like strains from humans (n = 8) and reptiles (n = 5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and genomic data from sap analysis, 16S rRNA gene and hsp60 sequence comparison, pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, DNA-DNA hybridization and whole genome sequencing demonstrated that these strains are closely related to C. fetus but clearly differentiated from recognized subspecies of C. fetus. Therefore, this unique cluster of 13 strains represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427(T) ( = ATCC BAA-2539(T) = LMG 27499(T)) as the type strain. Although this novel taxon could not be differentiated from C. fetus subsp. fetus and C. fetus subsp. venerealis using conventional phenotypic tests, MALDI-TOF MS revealed the presence of multiple phenotypic biomarkers which distinguish Campylobacter fetus subsp. testudinum subsp. nov. from recognized subspecies of C. fetus.",

keywords = "Amplified Fragment Length Polymorphism Analysis, Animals, Bacterial Typing Techniques, Campylobacter fetus, DNA, Bacterial, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S, Reptiles, Sequence Analysis, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization",

author = "Collette Fitzgerald and Tu, \{Zheng Chao\} and Mary Patrick and Tracy Stiles and Lawson, \{Andy J\} and Monica Santovenia and Gilbert, \{Maarten J\} and \{van Bergen\}, Marcel and Kevin Joyce and Janet Pruckler and Steven Stroika and Birgitta Duim and Miller, \{William G\} and Vladimir Loparev and Sinnige, \{Jan C\} and Fields, \{Patricia I\} and Tauxe, \{Robert V\} and Blaser, \{Martin J\} and Wagenaar, \{Jaap A\}",

year = "2014",

month = sep,

doi = "10.1099/ijs.0.057778-0",

language = "English",

volume = "64",

pages = "2944--2948",

journal = "International journal of systemic and evolutionary microbiology",

issn = "1466-5026",

publisher = "Microbiology Society",

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}

Fitzgerald, C, Tu, ZC, Patrick, M, Stiles, T, Lawson, AJ, Santovenia, M, Gilbert, MJ, van Bergen, M, Joyce, K, Pruckler, J, Stroika, S, Duim, B, Miller, WG, Loparev, V, Sinnige, JC, Fields, PI, Tauxe, RV, Blaser, MJ & Wagenaar, JA 2014, 'Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles', International journal of systemic and evolutionary microbiology, vol. 64, no. Pt 9, pp. 2944-2948. https://doi.org/10.1099/ijs.0.057778-0

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T1 - Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles

AU - Fitzgerald, Collette

AU - Tu, Zheng Chao

AU - Patrick, Mary

AU - Stiles, Tracy

AU - Lawson, Andy J

AU - Santovenia, Monica

AU - Gilbert, Maarten J

AU - van Bergen, Marcel

AU - Joyce, Kevin

AU - Pruckler, Janet

AU - Stroika, Steven

AU - Duim, Birgitta

AU - Miller, William G

AU - Loparev, Vladimir

AU - Sinnige, Jan C

AU - Fields, Patricia I

AU - Tauxe, Robert V

AU - Blaser, Martin J

AU - Wagenaar, Jaap A

PY - 2014/9

Y1 - 2014/9

N2 - A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetus-like strains from humans (n = 8) and reptiles (n = 5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and genomic data from sap analysis, 16S rRNA gene and hsp60 sequence comparison, pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, DNA-DNA hybridization and whole genome sequencing demonstrated that these strains are closely related to C. fetus but clearly differentiated from recognized subspecies of C. fetus. Therefore, this unique cluster of 13 strains represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427(T) ( = ATCC BAA-2539(T) = LMG 27499(T)) as the type strain. Although this novel taxon could not be differentiated from C. fetus subsp. fetus and C. fetus subsp. venerealis using conventional phenotypic tests, MALDI-TOF MS revealed the presence of multiple phenotypic biomarkers which distinguish Campylobacter fetus subsp. testudinum subsp. nov. from recognized subspecies of C. fetus.

AB - A polyphasic study was undertaken to determine the taxonomic position of 13 Campylobacter fetus-like strains from humans (n = 8) and reptiles (n = 5). The results of matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS and genomic data from sap analysis, 16S rRNA gene and hsp60 sequence comparison, pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, DNA-DNA hybridization and whole genome sequencing demonstrated that these strains are closely related to C. fetus but clearly differentiated from recognized subspecies of C. fetus. Therefore, this unique cluster of 13 strains represents a novel subspecies within the species C. fetus, for which the name Campylobacter fetus subsp. testudinum subsp. nov. is proposed, with strain 03-427(T) ( = ATCC BAA-2539(T) = LMG 27499(T)) as the type strain. Although this novel taxon could not be differentiated from C. fetus subsp. fetus and C. fetus subsp. venerealis using conventional phenotypic tests, MALDI-TOF MS revealed the presence of multiple phenotypic biomarkers which distinguish Campylobacter fetus subsp. testudinum subsp. nov. from recognized subspecies of C. fetus.

KW - Amplified Fragment Length Polymorphism Analysis

KW - Animals

KW - Bacterial Typing Techniques

KW - Campylobacter fetus

KW - DNA, Bacterial

KW - Humans

KW - Molecular Sequence Data

KW - Nucleic Acid Hybridization

KW - Phylogeny

KW - RNA, Ribosomal, 16S

KW - Reptiles

KW - Sequence Analysis, DNA

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

U2 - 10.1099/ijs.0.057778-0

DO - 10.1099/ijs.0.057778-0

M3 - Article

C2 - 24899653

SN - 1466-5026

VL - 64

SP - 2944

EP - 2948

JO - International journal of systemic and evolutionary microbiology

JF - International journal of systemic and evolutionary microbiology

IS - Pt 9

ER -

Campylobacter fetus subsp. testudinum subsp. nov., isolated from humans and reptiles

Abstract

Keywords

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